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FCMAB250A4 Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor®488, clone AW604

This Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.

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Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BCL2, Bcl-2 UniProt Number: P10415
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FCMAB250A4
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberFCMAB250A4
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-Bcl-2 Antibody, Alexa Fluor®488, clone AW604
      Background InformationBcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 also regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases. It appears to Inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Further understanding in how Bcl-2 functions and interacts with other proteins to promote or inhibit apoptosis may lead to new treatments for cancer, neurological disorders and autoimmune diseases.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Jurkat Cells
      PresentationPurified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to human Bcl-2
      CloneAW604
      HostMouse
      SpecificityAntibody recognizes Bcl-2.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
      Gene Symbol
      • BCL2
      • Bcl-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

      SIZE: 239 amino acids; 26266 Da

      SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.

      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

      TISSUE SPECIFICITY: Expressed in a variety of tissues.

      DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.

      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.

      DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

      SIMILARITY: Belongs to the Bcl-2 family
      Molecular Weight26 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaulated by flow cytometery using Jurkat cells.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      FCMAB250A4 04053252290091

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies