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MGAMMAG-300E MILLIPLEX® MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Isotyping
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MGAMMAG-300E
96-well plate  96 Well Plate
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      Übersicht

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      IgE MMplexLuminex xMAP
      Description
      Catalogue NumberMGAMMAG-300E
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay
      Background InformationFound only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcɛRI,
      found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and
      leukotrienes, resulting in an “allergic reaction”.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationSingle Plex
      Precision, %
      • Intra-Assay < 5%
      • Inter-Assay < 10%
      Panel TypeMAGNETIC Immune Response
      Quality LevelMQ200
      Applications
      ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This kit may be used for the analysis of Mouse IgE in Mouse serum, plasma, tissue/cell lysates and tissue culture supernatant samples.
      • The assay can be performed in less than 3 hours
      • This assay utilizes as little as 2uL of sample (a 1:200 dilution for plasma or serum is recommended; 2uL in 398uL of Assay Buffer). Tissue/ cell culture supernatants are diluted to approximately 1μg/mL in Assay Buffer
      Biological Information
      SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
      Species Reactivity
      • Mouse
      Analytes Available
      • IgE
      Accuracy
      • 82%
      Physicochemical Information
      Sensitivity
      • 0.16 ng/mL
      Standard Curve Range
      • 0.8 – 3500 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 Well Plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MGAMMAG-300E 04055977291155