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HCYTA-60K-PX48 MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay

The Human Cytokine/Chemokine/Growth Factor Panel A bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 48 multiple cytokine, chemokine, and growth factor biomarkers in human serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Growth Factors & Receptors, Oncology, Obesity, Metabolic Disorders
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HCYTA-60K-PX48
96-well plate  96 well plate
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      Übersicht

      Replacement Information

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      This panel is available for custom premix. Talk to your Protein Specialist today!

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      sCD40L EGF Eotaxin FGF-2 Flt-3 ligand Fractalkine G-CSF GM-CSF GROα IFNα2 IFNγ IL-1α IL-1β IL-1ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8 IL-9 IL-10 IL-12 (p40) IL-12 (p70) IL-13 IL-15 IL-17A IL-17E/IL-25 IL-17F IL-18 IL-22 IL-27 IP-10 MCP-1 MCP-3 M-CSF MDC (CCL22) MIG MIP-1α MIP-1β PDGF-AA PDGF-AB/BB RANTES TGFα TNFα TNFβ VEGF-A HMplexLuminex xMAP
      Description
      Catalogue NumberHCYTA-60K-PX48
      ReplacesHCYTMAG-60K-PX38 and HCYTMAG-60K-PX41
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay
      OverviewMILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

      Each MILLIPLEX® MAP panel and kit includes:
      • Quality controls (QCs) to qualify assay performance
      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
      • Optimized serum matrix to mimic native analyte environment in serum and plasma samples
      • Detection antibody cocktails designed to yield consistent analyte profiles within panel

      In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
      Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses.“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis.

      Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer.
      References
      Product Information
      Detection methodLuminex xMAP
      HS Code3002 15 90
      ConfigurationPremixed
      Precision, %
      • Intra-assay: <15%
      • Inter-assay: <20%
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      ApplicationThe Human Cytokine/Chemokine/Growth Factor Panel A bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 48 multiple cytokine, chemokine, and growth factor biomarkers in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight or two-hour incubation assay.
      • This assay requires 25 µL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.
      • This kit may be used for the analysis of all of the Human Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
      • RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
      • This premixed panel comes with a RANTES bead, so please plan your samples accordingly.
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • sCD40L
      • EGF
      • Eotaxin
      • FGF-2
      • Flt-3 ligand
      • Fractalkine
      • G-CSF
      • GM-CSF
      • GROα
      • IFNα2
      • IFNγ
      • IL-1α
      • IL-1β
      • IL-1ra
      • IL-2
      • IL-3
      • IL-4
      • IL-5
      • IL-6
      • IL-7
      • IL-8
      • IL-9
      • IL-10
      • IL-12 (p40)
      • IL-12 (p70)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17E/IL-25
      • IL-17F
      • IL-18
      • IL-22
      • IL-27
      • IP-10
      • MCP-1
      • MCP-3
      • M-CSF
      • MDC (CCL22)
      • MIG
      • MIP-1α
      • MIP-1β
      • PDGF-AA
      • PDGF-AB/BB
      • RANTES
      • TGFα
      • TNFα
      • TNFβ
      • VEGF-A
      Cross ReactivityThere was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
      Accuracy
      • 92% - 106%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
      Standard Curve Range
      • MIP-1β: 0.38 - 6,000 pg/mL
      • IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-18, MDC: 0.64 - 10,000 pg/mL
      • FLT-3L: 0.96 - 15,000 pg/mL
      • GROα, IFNγ, IL-3, IL-17A, RANTES, TGFα: 1.3 - 20,000 pg/mL
      • IL-1β, IL-1RA, TNFβ: 1.6 - 25,000 pg/mL
      • GM-CSF, IL-10, IP-10, VEGF-A: 2.6 - 40,000 pg/mL
      • EGF, Eotaxin, IL-12 (p70), IL-15, MCP-1, MIP-1α: 3 - 50,000 pg/mL
      • G-CSF, IL-1α: 4.8 - 75,000 pg/mL
      • IL-12 (p40), IL-13, MIG, TNFα: 6.4 - 100,000 pg/mL
      • IFNα2, MCP-3: 8 - 125,000 pg/mL
      • PDGF-AB/BB: 9.6 - 150,000 pg/mL
      • sCD40L, IL-22, PDGF-AA: 13 - 200,000 pg/mL
      • IL-27: 16 - 250,000 pg/mL
      • FGF-2: 26 - 400,000 pg/mL
      • Fractalkine, IL-17F: 32 - 500,000 pg/mL
      • IL-17E/IL-25, M-CSF: 40 - 625,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      HCYTA-60K-PX48 04061841058601

      Documentation

      MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Technische Informationen

      Titel
      Quality Control Range Sheet- A60MG-104 and A60MG-204
      Quality Control Range Sheet- A60MG-105 and A60MG-205
      Quality Control Range Sheet- A6602-106 and A6602-206
      Quality Control Range Sheet- A6602-106 and A6602-206
      Quality Control Ranges: Human Cytokine/ Chemokine/Growth Factor Magnetic Bead Panel A