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GCYT1-16K-PX12 MILLIPLEX® Chicken Cytokine/Chemokine premixed 12-plex Panel 1 - Immunology Multiplex Assay

The Chicken Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 12 cytokine and chemokine biomarkers in chicken serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Oncology, Obesity, Metabolic Disorders
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GCYT1-16K-PX12
96-well plate  96 well plate
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      This panel is available for custom premix. Talk to your Protein Specialist today!

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
      IL-16 IFN-α IFNγ IL-10 IL-21 IL-2 M-CSF IL-6 MIP-3α MIP-1β RANTES VEGF ChMplexNote: The protocol recommends a 1:4 dilution of serum and plasma samples. Luminex xMAP
      Description
      Catalogue NumberGCYT1-16K-PX12
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Chicken Cytokine/Chemokine premixed 12-plex Panel 1 - Immunology Multiplex Assay
      OverviewEMD Millipore’s MILLIPLEX® Chicken Cytokine/Chemokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in chicken serum, plasma and PBMC samples: Interferon alpha (IFNα), Interferon gamma (IFNγ), Interleukin 2 (IL-2), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 16 (IL-16), Interleukin 21 (IL-21), Macrophage Inflammatory protein-1β (MIP-1b), Macrophage Inflammatory protein-3 (MIP-3a), RANTES (regulated on activation, normal T cell expressed and secreted), Macrophage colony-stimulating factor (M-CSF), Vascular endothelial growth factor (VEGF). Note: The protocol recommends a 1:4 dilution of serum and plasma samples.

      MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

      Each MILLIPLEX® panel and kit includes:
      • Quality controls (QCs) to qualify assay performance
      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
      • Optimized serum matrix to mimic native analyte environment in serum and plasma samples
      • Detection antibody cocktails designed to yield consistent analyte profiles within panel

      The MILLIPLEX® Chicken Cytokine/Chemokine Panel thus enables you to focus on the therapeutic potential of cytokines and chemokines.
      Background InformationInflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for chicken as it is for humans. Both experience immunological diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokines in chicken has been limited primarily to ELISAs. Researchers now have the ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX® Chicken Cytokine/Chemokine Panel 1, based on the Luminex® xMAP® technology.
      References
      Product Information
      Detection methodLuminex xMAP
      HS Code3002 15 90
      ConfigurationPremixed
      Precision, %
      • Intra-assay: <10%
      • Inter-assay: <20%
      Multiplex Ordering RequirementsNote: The protocol recommends a 1:4 dilution of serum and plasma samples.
      Panel TypeMAGNETIC Cytokines/Chemokines
      Applications
      ApplicationThe Chicken Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 12 cytokine and chemokine biomarkers in chicken serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This assay may be run overnight (16-18 hours) at 2-8°C or for 2 hours at room temperature (20-25°C) with agitation on a plate shaker
      • A maximum of 25 μL per well 1:4 serum or plasma diluted in Serum Matrix should be used for all analytes. Tissue culture supernatants may require dilution in appropriate control medium. Tissue/cell extracts should be prepared in neutral buffers containing reagents and conditions that do not interfere with assay performance.
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Chicken
      Analytes Available
      • IL-16
      • IFN-α
      • IFNγ
      • IL-10
      • IL-21
      • IL-2
      • M-CSF
      • IL-6
      • MIP-3α
      • MIP-1β
      • RANTES
      • VEGF
      Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
      Accuracy
      • 87% - 100% using the overnight protocol
      • 95-100% using the 2 hour protocol
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual cytokines and chemokines.
      Standard Curve Range
      • IL-16: 7.32 - 30,000 pg/mL
      • IFNα: 3.66 – 15,000pg/mL
      • IFNγ: 18.3 – 75,000pg/mL
      • IL-10: 24.4 – 100,000pg/mL
      • IL-21: 36.6 – 150,000pg/mL
      • IL-2: 48.8 – 200,000pg/mL
      • M-CSF: 9.75 – 40,000pg/mL
      • IL-6: 122.1 – 500,000pg/mL
      • MIP-3α: 18.3 – 75,000pg/mL
      • MIP-1β: 4.88 – 20,000pg/mL
      • RANTES: 12.21 – 50,000pg/mL
      • VEGF: 4.88 – 20,000pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      GCYT1-16K-PX12 04065265239783