Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
SCC624
Sigma-AldrichLacun.3 Mouse Lung Adenocarcinoma Cell Line
Lacun.3 cells are capable of forming spheroids that can be maintained over multiple passages and display increased cancer stem cell characteristics including increased ALDH activity.
More>>Lacun.3 cells are capable of forming spheroids that can be maintained over multiple passages and display increased cancer stem cell characteristics including increased ALDH activity. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
SCC624
Description
Lacun.3 Mouse Lung Adenocarcinoma Cell Line
Alternate Names
Lacun-3
Lacun3
Background Information
Recent cancer research often studies a newly characterized type of cell referred to as cancer stem cells (CSCs) or also sometimes termed tumor-initiating cells (TICs.) These cells appear to have significant capabilities to grow, differentiate, generate tumors and resist chemotherapy. Tumor sphere cultures (TSCs) have emerged as a new format of studying cancer as well as understanding CSCs. The spheroid structures of TSCs appear to induce stem-like characteristics evidenced by the increased expression of stem cell markers. This 3D spheroid structure is also a much more accurate way to mimic the behavior of a solid tumor which develops in 3-dimensions and behaves quite differently from a monolayer cell culture. Spheroids are understood to have more tumor-forming capabilities when compared to monolayer cultures, yet the underlying mechanism for these characteristics is still under study. Stem-cell like properties, such as increased aldehyde dehydrogenase activity (ALDH), have already been used to identify CSCs in a variety of cancer types.
The Lacun.3 cell line was obtained from a chemically induced lung adenocarcinoma which developed in mice.1 This cell line is capable of forming spheroids when cultured in the appropriate media. The spheroid culture can be maintained over multiple passages and displays increased stem-cell characteristics including increased ALDH activity when compared to adherent cultures using the same line. TSC cultured Lacun.3 shows high tumor formation when injected into mice although metastatic capabilities appear to be weakened. While TSC Lacun.3 growth characteristics are slower compared to adherent culture, their chemoresistance capabilities were higher. In addition, a drug that was screened to specifically kill CSCs had much higher success in killing TSC Lacun.3 cells, indicating their CSC phenotype. Furthermore, the Lacun.3 cell line provides a useful model for studying CSCs by spheroid formation.
Source Non-GMO. Cell line is derived from a chemically induced lung adenocarcinoma developed in a mouse model.
References 1. Bleau A-M, Zandueta C, Redrado M, Martínez-Canarias S, Larzábal L, Montuenga LM, Calvo A, Lecanda F. 2015. Sphere-derived tumor cells exhibit impaired metastasis by a host-mediated quiescent phenotype. Oncotarget. 6(29):27288–27303. doi:https://doi.org/10.18632/oncotarget.4803. [accessed 2024 Apr 11]. 2. Weiswald L-B, Bellet D, Dangles-Marie V. 2015. Spherical Cancer Models in Tumor Biology. Neoplasia. 17(1):1–15. 3. Toledo-Guzmán ME, Hernández MI, Gómez-Gallegos ÁA, Ortiz-Sánchez E. 2019. ALDH as a Stem Cell Marker in Solid Tumors. Current Stem Cell Research & Therapy. 14(5):375–388.
References
Product Information
Components
≥1x106 viable Lacun.3 Cells (Catalog No. SCC624). Store in liquid nitrogen.
Lacun.3 cells are capable of forming spheroids that can be maintained over multiple passages and display increased cancer stem cell characteristics including increased ALDH activity.
Key Applications
Cell Culture
Application Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
Host
Mouse
Cell Line Type
Cancer Cells
Lung cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
• Each vial contains ≥ 1X106 viable cells. • Cells are tested negative for infectious diseases by a Essential CLEAR Panel by Charles River Animal Diagnostic Services. • Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services • Cells are negative for mycoplasma contamination.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Lacun.3 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.