Millipore Sigma Vibrant Logo

QIA33 FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

View Products on Sigmaaldrich.com
QIA33
Preis & Verfügbarkeit

Übersicht

Key Spec Table

Detection Methods
Colorimetric

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
QIA33-1EA
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Pappschachtel 1 ea
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      Bitte erfragen
      Description
      OverviewNon-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Terminal deoxynucleotidyl transferase (TdT) binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.
      Catalogue NumberQIA33
      Brand Family Calbiochem®
      SynonymsTUNEL Assay
      Materials Required but Not Delivered xylene
      ethanol, 100, 90, 80, 70%
      methanol
      30% hydrogen peroxide
      tris-buffered saline (1X TBS, 20 mM Tris pH 7.6, 140 mM NaCl)
      10 mM Tris pH 8
      1 mM MgSO4 in 1X TBS (optional, for use in generating positive control)
      DNaseI (optional, for use in generating positive control)
      distilled water
      coplin jars, glass or plastic with slide holders
      wash bottle or beaker for rinsing slides
      humidified chamber
      glass coverslips
      mounting media (such as Permount®)
      microscope
      2-20 µl, 20-200 µl, and 200-1000 µl precision pipettors with disposable tips
      microcentrifuge tubes
      parafilm®
      absorbent wipes
      ice
      References
      ReferencesXiao, D. and Bullock, R. 1996. J. Neurosurgery 85, 655.
      Gu, Y., et al. 1994. Endocrinology 135, 1272.
      Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493.
      Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437.
      Martin, S.J., et al. 1990. J. Immunology 145, 1859.
      Wyllie, A. H. 1980. Nature 284, 555.
      Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.
      Product Information
      Detection methodColorimetric
      Form50 Tests
      FormatMicroscopy
      Kit containsCounterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Paraffin Sections
      Biological Information
      Assay time4 h
      Sample TypeFrozen or paraffin sections or fixed-cell preparations
      Species Reactivity
      • A Broad Range Of Species
      Safety Information
      R PhraseR: 25-36/37/38-42-52/53

      Toxic if swallowed.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
      S PhraseS: 24-26-3/7-36/37/39-45-61

      Avoid contact with skin.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Keep container tightly closed in a cool place.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Avoid release to the environment. Refer to special instructions/safety data sheet.
      Product Usage Statements
      Intended useThe Calbiochem® TdT-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, and in cell preparations fixed on slides.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C in a non-frostfree freezer. Following initial thaw store the Stop Buffer and Methyl Green Counterstain at room temperature and the Blocking Buffer at 4°C. Refreezing of these components, however, does not appear to affect their performance.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCounterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.
      Global Trade Item Number
      Bestellnummer GTIN
      QIA33-1EA 07790788054038