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344146 FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

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344146
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344146-100UG
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      Kst.-Ampulle 100 μg
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      Description
      OverviewRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
      Catalogue Number344146
      Brand Family Calbiochem®
      SynonymsFTase, Farnesyl Proteintransferase
      References
      ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
      Reiss, Y., et al. 1990. Cell 62, 81.
      Product Information
      FormLiquid
      FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationFPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
      Biological Information
      Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
      Purity≥90% by SDS-PAGE
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Bestellnummer GTIN
      344146-100UG 04055977215236