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Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
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Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
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17-10495
Sigma-AldrichEZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes
The EZ-Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins.
More>>The EZ-Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes
Overview
Features and Advantages
Recover non-coding RNA containing complexes using lncRNA and other types of chromatin associated RNA as targets
Identify sites of genomic interaction of chromatin associated RNA
Perform proteomic analysis of chromatin associated RNA regulatory complexes
Includes Streptavidin magnetic beads, plus all key buffers, enzymes, and reagents
All kits include a negative control probe set and a detailed protocol
EZ-Magna ChIRP kit includes a biotinylated TERC lncRNA positive control probe set and qPCR and RT-qPCR detection primer sets.
Biotinylated capture probe sets for lncRNA TERC and NEAT1 sets are also available separately
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.
Learn more about the ChIRP method in JOVE! (Watch Now!)
Cells, stimulated or treated as needed for the experimental system
PBS (RNase free)
Glutaraldehyde solution
Formamide
Anti-sense oligo probes against chromatin associated RNA of interest. Note that pre-designed ChIRP probe sets are available for NEAT1 lncRNA (cat# 03-308) and TERC lnRNA (cat# 03-309).
Reagents for RNA-seq Library Construction -if performing RNA-seq analysis
Reagents for DNA-seq library constructionif performing DNA-seq analysis (e.g. PureGenome™ Low Input NGS Library Construction Kit, Cat. # 17-10492
Additional common laboratory reagents (water, 100% ethanol, chloroform etc.)
Equipment
Sonicator
Hybridization equipment
Magnetic Separation Rack (e.g. Magna GrIP™ Rack; 8 well, EMD Millipore Cat. # 20-400 or PureProteome™ Magnetic Stand, EMD Millipore Cat. # LSKMAGS08 and LSKMAGS15
Vortex mixer
Rotating wheel/platform
Centrifuge for cell culture
Microcentrifuge
Thermomixer® (60°C capable)
Variable temperature water bath or incubator
Rotating microtube mixer
Real-time PCR thermal cycler
NanoDrop™ or spectrophotometer
Microfuge tubes, pipettes, tips and other common plastic disposables
References
Product Information
Components
10X Glycine
Lysis Buffer
Hybridization Buffer (without 15% formamide)
Wash Buffer
Proteinase K Buffer (for DNA)
Proteinase K Buffer (for RNA)
DNA Elution Buffer
0.5M EDTA
Streptavidin Magnetic Beads
RNase Inhibitor
Protease Inhibitor Cocktail III
Proteinase K Solution
DNase I RNase free
DNase I Reaction Buffer
RNase A (20 mg/mL)
RNase H (10U/ µL)
Magna ChIRP™ Negative Control Probe Set (LacZ)
Magna ChIRP™ TERC lncRNA Probe Set (Even)
Magna ChIRP™ TERC lncRNA Probe Set (Odd)
RNA Positive Control Primers (TERC Gene)
RNA Negative Control Primers (GAPDH Gene)
Magna ChIRP™ Primers, WNT-1 precursor
ChIP Primers, GAPDH coding D2
Presentation
Three boxes containing key reagents for the extraction of ChIRP ready chromatin, plus magnetic beads, enzymes, buffers, biotinlyated negative control probes, biotinylated TERC odd and even positive control probe set and PCR primers to allow the performance of 12 chromatin isolation by RNA purification assays.
The EZ-Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins.
Key Applications
DNA-binding Proteins
RNA Detection
DNA Sequencing
Protein Determination
Protein Interaction Assays
Purification with magnetic beads
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Packaging Information
Material Size
12 Assays
Material Package
Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes, even and odd positive control TERC lncRNA probes, and control PCR primer sets)
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
17-10495
04055977127065
Documentation
EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes SDB
Despite great advances have been made in the understanding of biology of osteosarcoma, the molecular mechanisms involved in osteosarcoma tumorigenesis and progression are still largely unknown. Long noncoding RNA (lncRNA) is a new type of RNA molecule, which plays pivotal roles in many tumors. lncRNA BCAR4 has been identified as an oncogenetic lncRNA involved in the progression of breast cancer. However, the functions and clinical significances of BCAR4 in osteosarcoma are unknown now. In this study, we found that BCAR4 was significantly upregulated in osteosarcoma tissues. Increased expression of BCAR4 was significantly correlated with large tumor size, advanced Enneking stage, lung metastasis, and poor prognosis. Functional experiments demonstrated that knockdown of BCAR4 inhibits the proliferation and migration of osteosarcoma cell in vitro. Consistently, knockdown of BCAR4 inhibits osteosarcoma tumorigenesis and lung metastasis in vivo. Chromatin isolation by RNA purification assay showed that BCAR4 physically associates with the promoters of GLI2 target genes. The depletion of BCAR4 inhibits the expression of GLI2 target genes and GLI2 reporter luciferase activity in a dose-dependent manner. The expression of BCAR4 and GLI2 target genes is significantly correlated in osteosarcoma tissues. Depletion of DLI2 abolished the effects of BCAR4 on osteosarcoma. Taken together, these findings demonstrated that BCAR4 promotes osteosarcoma progression via activating GLI2-dependent gene transcription and serves as a potential prognostic biomarker and a therapeutic target of osteosarcoma.
