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17-10078 EZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

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Research Category: Epigenetics & Nuclear Function
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17-10078
96 assays  Kit capacity: 96 chromatin immunoprecipitation assays
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      Replacement Information
      Description
      Catalogue Number17-10078
      Brand Family Upstate
      Trade Name
      • EZ-Magna ChIP
      • Upstate
      DescriptionEZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit
      OverviewThe EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative control antibodies and validated primer set plus a detailed protocol.

      Features & Benefits
      • Complete set of materials for up to 96 ChIP reactions
      • Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
      • Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
      • Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
      • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
      • Protocols for using cells or tissues
      • Direct analysis of DNA without additional clean-up steps
      • Compatible with ChIPAb+ validated antibody and primer sets
      Materials Required but Not DeliveredMagna Grip HT96™ Rack 96-well magnetic separation stand (Cat.# 17-10071) or similar 96-well magnetic rack.
      References
      Product Information
      Components
      • Magna ChIP protein A/G magnetic beads
      • HT96 Nuclei Isolation Buffer
      • HT96 ChIP Buffer (Sonication/ChIP/Wash)
      • Low Stringency IP Wash Buffer
      • HT96 ChIP Elution Buffer
      • Proteinase K Solution
      • Protease Inhibitor Cocktail III
      • 10X Glycine
      • 10X PBS
      • 96 Well ChIP Plate
      • 96 Well Thermal Plate
      • Plate Seal
      • Strip Caps
      • Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody
      • Normal Rabbit IgG -negative control antibody
      • GAPDH Primers - PCR positive control
      PresentationTwo boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.
      Quality LevelMQ100
      Applications
      ApplicationThe EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
      Packaging Information
      Material Size96 assays
      Material PackageKit capacity: 96 chromatin immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      17-10078 04053252012136

      Documentation

      Broschüre

      Titel
      An Introduction to Antibodies and Their Applications
      Magna ChIP™ HiSens Kits
      New Products: Volume 3, 2012

      Technische Informationen

      Titel
      96 well Template 2 Up
      96 well Template Single
      Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      Datenblatt

      Titel
      Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation.

      Gebrauchsanleitungen

      Titel
      EZ-Magna ChIP™ HT96 (Cat. No. 17-10078)