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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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The ESGRO®-2i Supplement Kit (1000x) contains LIF a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.
More>>The ESGRO®-2i Supplement Kit (1000x) contains LIF a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state. Less<<
ESGRO®-2i Supplement Kit (1000x): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO supplement is supplied 0.22 micron filtered, and tested negative in mycoplasma tests. Endotoxin level is less than 0.1 ng per µg of LIF.
Description
Catalogue Number
ESG1121
Brand Family
Chemicon®
Trade Name
ESGRO
Chemicon
Description
ESGRO®-2i Supplement Kit (1000x)
Alternate Names
2i Naive ES/IPS Supplement
Background Information
Two stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs. Mouse Leukemia Inhibitory Factor (LIF) is a critical component in mouse ES cell culture media where it promotes the self-renewal and maintains the pluripotency of mouse embryonic stem (ES) cells. Recent studies have determined that inhibitors that block MAPK/Erk pathway, in combination with the glycogen synthase kinase 3 (GSK3) inhibitor, protect mouse ES cells from differentiation-inducing signals, allowing for self-renewal even in the absence of LIF. Mouse ES cells cultured in these conditions maintain their LIF responsiveness and studies have shown that adding LIF to the 2i culture conditions led better colony propagation and enhanced the general health of ES colonies. Supports a naive/ground pluripotent state.
References
Product Information
Components
ESGRO® Supplement: (Part No. CS210511): Mouse recombinant Leukemia Inhibitory Factor (LIF), 0.5 x 106U/mL. One (1) 0.5 mL vial.
MEK/GS3 Inhibitor Supplement: (Cat. No. CS210510-500UL): One (1) 0.5 mL vial.
Presentation
Both supplements were formulated at 1000x concentration in solution. Mouse LIF is prepared in phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability. MEK/GSK3 inhibitor supplement is prepared in DMSO.
The ESGRO®-2i Supplement Kit (1000x) contains LIF a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.
Key Applications
Stem Cell Culture
Application Notes
Suggested Protocol for Medium Preparation: 1. Thaw MEK/GSK3 inhibitor supplement and mLIF at room temperature. 2. Each vial of supplement is sufficient to make 500 mL of complete medium for serum-free or serum dependent mouse ES culture. 3. Aseptically transfer the contents of the vials containing the supplements at 1000x dilution to the pre-warmed bottle of serum free basal medium such as ESGRO Complete Basal Medium (Cat. No. SF002-500) or serum containing medium and mix. 4. The complete medium can be filtered through 0.22 µm filter (Cat. No. SCGVU05RE). 5. After supplements are added to the medium, store in the dark at 4°C for up to ten days.
Biological Information
Purity
The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO supplement is supplied 0.22 micron filtered, and tested negative in mycoplasma tests. Endotoxin level is less than 0.1 ng per µg of LIF.
Specific Activity
ESGRO Supplement is assessed both on mouse ES cells and on murine M1 myeloid leukemic cells. A standard of 50 Units is defined as the concentration of ESGRO supplement in 1.0 mL of tissue culture medium that induces the differentiation of 50% of M1 colonies (Metcalf, 1988).<br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL<br />Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Storage and Shipping Information
Storage Conditions
Store at -20°C. Upon thaw, ESGRO Supplement should be stored at 2-8°C. Do not refreeze ESGRO Supplement (part no. CS210511). Refer to label for expiration date.
Packaging Information
Material Size
1 kit per 500 mL medium
Material Package
Provided in ready to use aliquots sufficient for supplementing 500 mL mouse ES cell culture medium.
