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SCR029 Cryopreserved Mouse Cortical Neural Stem Cells

The Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice.

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Key Applications: Stem Cell Culture
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SCR029
1 vial  1 x 106 cells
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      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR029
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCryopreserved Mouse Cortical Neural Stem Cells
      OverviewMillipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

      We recommend that Millipore's Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population.

      For Research Use Only; not for use in diagnostic procedure.
      Materials Required but Not Delivered1. Neural Stem Cell Expansion Medium (Millipore Catalog No. SCM003)

      2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity

      > 2 x 10e6 Units/mg, Millipore Catalog No. GF003)

      3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Millipore Catalog No. GF001)

      4. Heparin (Sigma Catalog No. H-1027)

      5. Poly-L-ornithine (Sigma Catalog No. P3655)

      6. Laminin (Millipore Catalog No. CC095)

      7. ESGRO Complete™ Accutase™ (Millipore Catalog No. SF006)

      8. Tissue culture-ware

      9. Phosphate-Buffered Saline (1X PBS) (Millipore Catalog No. BSS-1005-B)

      10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

      11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

      12. Primary and secondary antibodies

      13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

      14. Anti-fading mounting solution (DABCO/PVA)

      15. Hemacytometer

      16. Microscope
      References
      Product Information
      Components
      • 1x10e6 viable Mouse Cortical Neural Stem Cells: (Part No. 2004115) derived from mixed gender C57/BL6 embryonic day 15-18 mice, cryopreserved. Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationThe Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at the recommended storage conditions (liquid nitrogen), mouse cortical neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
      Packaging Information
      Material Size1 vial
      Material Package1 x 106 cells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCR029 04053252723667