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HTS170M ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor

Human sst1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Host Cell: Chem-1 Uni Prot Number: P30872 Entrez Gene Number: NM_000868
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HTS170M
200 units  
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      Übersicht

      Replacement Information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1sst1
      Description
      Catalogue NumberHTS170M
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor
      OverviewHuman sst1
      Background InformationSomatostatin, a 14 or 28 amino acid peptide, inhibits hormone secretion from exocrine glands, and displays an antiproliferative activity on a number of cell types. A family of 5 Gi-coupled receptors mediates the effects of somatostatin. The classical activity of somatostatin of inhibiting growth hormone release from the pituitary appears to be mediated by sst1. In addition, sst1 functions as an inhibitory autoreceptor on somatostatin neurons in the hippocampus, hypothalamus, basal ganglia and retina (Thermos et al., 2006). The somatostatin receptors are targets for anticancer drugs and for treatment of hormonal dysfunction. Millipore's sst1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of sst1. The membrane preparations exhibit a Kd of 1.4 nM for [125I]- somatostatin-14. With 0.5 nM [125I]- somatostatin-14, 5 μg/well sst1 Membrane Prep yields greater than 12 fold signal-to-background ratio.
      References
      Product Information
      FormatMembranes
      PresentationOne package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 12-fold signal:background with [125I]- somatostatin-14 at 0.5 nM.

      Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
      Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
      Quality LevelMQ100
      Applications
      ApplicationHuman sst1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
      Key Applications
      • Radioligand binding assay
      • GTPγS binding
      Application NotesRadioligand binding assay and GTPγS binding
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • SSTR1
      GPCR ClassA
      Host CellsChem-1
      Protein Targetsst1
      Target Sub-FamilySomatostatin
      UniProt Number
      Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

      Binding buffer: 50 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
      Radioligand: [125I]- somatostatin-14 (PerkinElmer NEX389)
      Wash Buffer: 50 mM HEPES, pH 7.4, 500 mM NaCl, 0.1% BSA, filtered and stored at 4°C.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of sst1 membrane prep.
      Product Usage Statements
      5 µg/well
      Signal:Background 20.4
      Specific Binding (cpm) 14,647

      SPECIFICATIONS: 1 unit = 5 μg membrane preparation
      Bmax: 8.4 pmol/mg
      Kd: 1.4 nM
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
      Packaging Information
      Material Size200 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      HTS170M 04053252513367

      Documentation

      ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      ChemiSCREEN™ Membrane Preparation Recombinant Human sst1 Somatostatin Receptor Analysenzertifikate

      TitelChargennummer
      CHEMISCREEN MEMBRANE PREPARATION RECOMBINANT HUMAN sst1 SOMATOSTATIN RECEPTOR -
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN SST1 SOMATOSTATIN RECEPTOR - 2116643 2116643
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN SST1 SOMATOSTATIN RECEPTOR - 2202689 2202689

      Datenblatt

      Titel
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN SST1 SOMATOSTATIN RECEPTOR

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      Kategorien

      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations