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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-phospho NMDAR2A (Ser1459), Cat. No. ABN2251, is a rabbit polyclonal antibody that detects NMDAR2A phosphorylated on Serine 1459 and is tested for use in Immunocytochemistry and Western Blotting.
More>>Anti-phospho NMDAR2A (Ser1459), Cat. No. ABN2251, is a rabbit polyclonal antibody that detects NMDAR2A phosphorylated on Serine 1459 and is tested for use in Immunocytochemistry and Western Blotting. Less<<
Anti-GABA A Receptor γ2 Antibody, cytoplasmic loop
Übersicht
Replacement Information
Description
Catalogue Number
ABN2251-100UG
Description
Anti-phospho NMDAR2A (Ser1459)
Alternate Names
Glutamate receptor ionotropic NMDA 2A
GluN2A
Glutamate [NMDA] receptor subunit epsilon-1
N-methyl D-aspartate receptor subtype 2A
NR2A
hNR2A
Background Information
Glutamate receptor ionotropic, NMDA2A (UniProt: Q12879; also known as GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, hNR2A) is encoded by the GRIN2A (also known as NMDAR2A) gene (Gene ID: 2903) in human. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2A is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. NMDAR2A exists as a heterotetramer and the heterotetrameric channels are composed of two zeta subunits (GRIN1) and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro). It can also form heterotetrameric channels that contain at least one zeta subunit (GRIN1), at least one epsilon subunit, plus GRIN3A or GRIN3B. In vivo, the subunit composition may depend on the expression levels of the different subunits. These heterotetrameric, ligand-gated ion channels display high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by magnesium ions. It has been shown that channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B. Mutations in GRIN2A gene are known to cause epilepsy, speech disorders, and mental retardation. Two isoforms of NMDAR2A have ben described that are produced by alternative splicing. (Ref.: Hess, SD. et al. (1996). J. Pharmacol. Exp. Therap. 278(2); 808-816).
References
Product Information
Format
Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.
Anti-phospho NMDAR2A (Ser1459), Cat. No. ABN2251, is a rabbit polyclonal antibody that detects NMDAR2A phosphorylated on Serine 1459 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Tested applications
Western Blotting Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Western Blotting applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).
Immunocytochemistry Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Immunocytochemistry applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
A peptide corresponding to 176 amino acids, phosphorylated on Serine 1459, from the C-terminal region of Human NMDAR2A.
Epitope
C-terminal
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects Phospho-NMDAR2A (Ser1459).
~200 kDa observed; 165.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lysates from wild-type HEK293T cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NMDAR2A phosphorylated on serine 1459 in lysate from HEK293T cells, but not in lysates from cells transfected with S1459A NMDAR2A.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
