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Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 is a Mouse Monoclonal Antibody for detection of dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) also known as Histone H3.1 & has been validated in WB, DB, ICC, IP & IHC.
More>>Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 is a Mouse Monoclonal Antibody for detection of dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) also known as Histone H3.1 & has been validated in WB, DB, ICC, IP & IHC. Less<<
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Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Conversely,deacetylation of histone H3 lysine 9 is associated with gene-activating events. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
References
Product Information
Format
Ascites
Control
Untreated and etoposide acid extract treated HeLa cell lysates.
Presentation
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 is a Mouse Monoclonal Antibody for detection of dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) also known as Histone H3.1 & has been validated in WB, DB, ICC, IP & IHC.
Key Applications
Western Blotting
Dot Blot
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry
Application Notes
Western Blot Analysis: A representative lot was used by an independent laboratory in KCl whole-cell extract. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Dot Blot Analysis: A representative lot was used by an independent laboratory in unmodified and modified Histone H3 peptides. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in NIH/3T3 cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse testis tissue. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Epitope
Dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Clone
51TA-2H1
Host
Mouse
Specificity
This antibody recognizes Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Evaluated by Western Blot in untreated and etoposide acid extract treated HeLa cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Histone H3 on 10 µg of untreated and etoposide acid extract treated HeLa cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Histone H3 tails containing dimethylated lysine and adjacent phosphorylated serine modifications adopt a specific conformation during mitosis and meiosis. Eberlin, Adrien, et al. Mol. Cell. Biol., 28: 1739-54 (2008)
2008
Condensation of chromatin, mediated in part by posttranslational modifications of histones, is essential for cell division during mitosis. Histone H3 tails are dimethylated on lysine (Kme2) and become phosphorylated on serine (Sp) residues during mitosis. We have explored the possibility that these double modifications are involved in the establishment of H3 tail conformations during the cell cycle. Here we describe a specific chromatin conformation occurring at Kme2 and adjacently phosphorylated S of H3 tails upon formation of a hydrogen bond. This conformation appears exclusively between early prophase and early anaphase of the mitosis, when chromatin condensation is highest. Moreover, we observed that the conformed H3Kme2Sp tail is present at the diplotene and metaphase stages in spermatocytes and oocytes. Our data together with results obtained by cryoelectron microscopy suggest that the conformation of Kme2Sp-modified H3 tails changes during mitosis and meiosis. This is supported by biostructural modeling of a modified histone H3 tail bound by an antibody, indicating that Kme2Sp-modified H3 tails can adopt at least two different conformations. Thus, the H3K9me2S10p and the H3K27me2S28p sites are involved in the acquisition of specific chromatin conformations during chromatin condensation for cell division.
