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ABE441 Anti-dimethyl Histone H4 (Arg3), Asymmetric Antibody

This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, HIST4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4 UniProt Number: P62805
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ABE441
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE441
      DescriptionAnti-dimethyl Histone H4 (Arg3), Asymmetric Antibody
      Alternate Names
      • Histone H4
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in HeLa cells.

      Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

      DyLight® is a registered trademark of Thermo Fisher Scientific.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4, asymmetrically dimethylated at Arg3.
      EpitopeN-terminus
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • HIST4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      Modifications
      • Methylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and CARM1 treated recombinant Histone H4.

      Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE441 04053252951961