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MAB1864-I Anti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2

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MAB1864-I
100 μg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Yeast, Porcine, ChWB, ICC, IHC, EM, ELISA, RIARPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1864-I
      DescriptionAnti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2
      Alternate Names
      • Tubulin alpha-1A chain
      • Alpha-tubulin 3
      • Tubulin B-alpha-1
      • Tubulin alpha-3 chain
      • alpha-Tubulin, tyrosinated
      Background InformationTubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast. Microtubules are dynamic protein filaments involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Microtubules are assembled from α- and β-tubulin subunits, both of which are subjected to posttranslational modifications, including the removal of the C-terminus aromatic amino acid from α-tubulin (tyrosine in mammals and phenylalanine in yeast) by carboxypeptidase actifvity. Because the C-terminus tyrosine/phenylalanine is preceded by glutamate (E) residues, the detyrosinated/dephenylalaninated α-tubulin is also referred to as Glu-tubulin. While tyrosine can be reattached to the C-terminus of the Glu-tubulin by tubulin-tyrosine ligase in mammals, phenylalanine is not added posttranslationally to the Glu-tubulin in yeast despite the presence of the yeast YBR094w gene that exhibits significant homology to tubulin-tyrosine ligase in other organisms.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry, Electron Microscopy, ELISA and Radioimmunoassay for the detection of alpha-Tubulin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Electron Microscopy
      • ELISA
      • Radioimmunoassay
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected tyrosinated alpha-tubulin in 10 µg of C2C12 cell lysate.
      Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected tyrosinated alpha-tubulin in A431, HeLa and HUVEC cells.
      Immunocytochemistry Analysis: Representative lots detected phenylalaninated alpha-tubulin immunoreactivity in yeast and tyrosinated alpha-tubulin immunoreactivity in HEK293 cells (Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582; Bandyopadhyay, B., et al. (2007). J Biol Chem. 282(22):16454-16464).
      Immunohistochemistry Analysis: A representative lot detected microtubules tyrosinated alpha-tubulin immunoreactivity in mid-streak level section of Hamburger-Hamilton stage 4 (HH4) chick embryos (Nakaya, Y., et al. (2008). Nat. Cell Biol. 10(7):765-775).
      Electron Microscopy Analysis: Representative lots detected microtubules phenylalaninated alpha-tubulin immunoreactivity using crude yeast nuclear envelope preparations and microtubules tyrosinated alpha-tubulin immunoreactivity in NIH/3T3 cells (Wehland, J., and Willingham, M.C. (1983). J Cell Biol. 97(5 Pt 1):1476-1490; Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582).
      ELISA Analysis: The antigenicity of clone YL1/2 was determined by competitive ELISA using pig brain-derived tubulin against alpha-tubulin peptides with or without Tyr/Phe at the C-terminal end (Wehland, J., et al. (1984). EMBO J. 3(6):1295-1300).
      Radioimmunoassay Analysis: The immunoreactivity of clone YL1/2 toward chick brain tubulin and yeast tubulin was determined by radioimmunoassays (Kilmartin J.V., et al. (1982). J Cell Biol. 93(3):576-582).
      Biological Information
      ImmunogenPurified yeast tubulin
      EpitopeC-terminus
      CloneYL 1/2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityReacts with alpha-tubulin with phenylalanine (yeast) or tyrosine (mammalian and chicken) at the C-terminus (PMID 6204858). Does not react toward posttranslationally modified alpha-tubulin lacking the C-terminus Phe or Tyr.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Yeast
      • Porcine
      • Chicken
      Species Reactivity NoteHuman, Mouse, Rat, Porcine, Bovine, Yeast, Chicken. Expected to react with a broad range of species based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBA1A
      • TUBA3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~55 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected tyrosinated alpha-tubulin in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Bestellnummer GTIN
      MAB1864-I 04055977288285