Millipore Sigma Vibrant Logo

ST1070 Anti-Tiam1 Rabbit pAb

This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.

Anti-Tiam1 Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-T-Cell Lymphoma Invasion and Metastasis 1

View Products on Sigmaaldrich.com
ST1070
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
ST1070-50UG
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 50 μg
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      Description
      OverviewRecognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
      Catalogue NumberST1070
      Brand Family Calbiochem®
      SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
      Application Data
      Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
      Product Information
      FormLiquid
      FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
      Positive controlJurkat or NIH3T3 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1070-50UG 04055977224405

      Documentation

      Anti-Tiam1 Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Tiam1 Rabbit pAb Analysenzertifikate

      TitelChargennummer
      ST1070

      Literatur

      Übersicht
      Mertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-October-2007 RFH
      SynonymsAnti-T-Cell Lymphoma Invasion and Metastasis 1
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
      BackgroundTiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
      IsotypeIgG
      Specieshuman, mouse
      Positive controlJurkat or NIH3T3 cells
      FormLiquid
      FormulationIn Tris-Citrate/phosphate, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Highly Toxic
      ReferencesMertens, A.E., et al. 2003. FEBS Lett. 546, 11.
      Qi, H., et al. 2001. Cell Growth Differ. 12, 603.
      Stam, J.C., et al. 1997. J. Biol. Chem. 272, 28447.