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ABS2269-25UG
Sigma-AldrichAnti-TRPM4
Anti-TRPM4, Cat. No. ABS2269, is a rabbit polyclonal antibody that detects TRPM4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
More>>Anti-TRPM4, Cat. No. ABS2269, is a rabbit polyclonal antibody that detects TRPM4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. Less<<
Transient receptor potential cation channel subfamily M member 4
Calcium-activated non-selective cation channel 1
Long transient receptor potential channel 4
TrpC-4
LTrpC4
MLS2s
Melastatin-like 2
Background Information
Transient receptor potential cation channel subfamily M member 4 (UniProt: Q9ESQ5; also known as Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, LTrpC-4, LTrpC4, MLS2s, Melastatin-like 2) is encoded by the Trpm4 (also known as Ltrpc4) gene (Gene ID: 171143) in rat. TRPM4 is a multi-pass membrane protein with four cytoplasmic domains; six transmembrane domains; and four extracellular domains. Two isoforms of TRPM4 have been described that are produced by alternative splicing. Isoform 1 is highly expressed in the testis tissue with a moderate expression in brain, spleen, and thymus. Isoform 2 is expressed in the brain and spleen. It serves as a calcium-activated non-selective cation channel that mediates membrane depolarization. Although it is a calcium activated channel, it is impermeable to calcium. It mediates the transport of monovalent cations that leads to membrane depolarization. It is reported to participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL-2 production. TRPM4 is also involved in myogenic constriction of cerebral arteries. It plays a role in control of insulin secretion in pancreatic β-cells. It may also be involved in pacemaking activity or could cause irregular electrical activity under conditions of Calcium overload. It is known to affect Th1 and Th2 cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. This polyclonal antibody is reported toblock the channel vua binding to a region close to the channel porefrom extracellular space. Bu blocking TRPM4, it can downregulate TRPM4 surface expression and prevent hypoxia-induced cell swelling. (Ref.: Wei, S., et al. (2020). Front Cell Dev Bio. 8; 562584; Chen, B;et al. (2019). Pflugers Arch. 471(11-12); 1455-1466; Guinamard, R., et al. (2006). Hypertension. 48(4); 587-594; Launay, P., et al. (2002). Cell. 109(3); 397-407).
References
Product Information
Format
Purified
Control
MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94000+ and ever-growing portfolio of antibodies to meet your research needs in Apoptosis and cancer
Cell structure
Cell signaling
Epigenetics
Inflammation
Immunology
Neuroscience
Stem cell biology
and more. Visit our Antibody Explorer Tool to view antibodies by clonality
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Presentation
Purified rabbit polyclonal antibody in PBS without azide.
Anti-TRPM4, Cat. No. ABS2269, is a rabbit polyclonal antibody that detects TRPM4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Application Notes
Tested applications
Immunocytochemistry Analysis: A representative lot detected TRPM4 in Immunocytochemistry application (Low, S.W., et. al. (2021). Sci Rep. 11(1):10411).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected TRPM4 in rat spinal cord tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
GST-conjugated linear peptide corresponding to 28 amino acids between transmembrane segment 5 and 6 from the C-terminal half of rat TRPM4.
Epitope
C-terminal extracellular half
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Transient receptor potential cation channel subfamily M member 4 (TRPM4). It targets an epitope within 24 amino acids from the C-terminal, extracellular domain.
~135 kDa observed; 135.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lysate from HEK293 cells transfected with Mouse TRPM4.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRPM4 in lysate from HEK293 cells transfected with Mouse TRPM4.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
