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Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.
More>>Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2156-100UL
Description
Anti-TLR9 Antibody, clone NaR9
Alternate Names
Toll-like receptor 9
CD289
Background Information
Toll-like receptor 9 (UniProt: Q9EQU3; also known as CD289) is encoded by the Tlr9 gene (Gene ID: 81897) in murine species. TLR9 is a single-pass type I membrane protein that serves as an innate immune sensor that recognizes microbial DNA. It exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand. TLR9 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 26-818), a short transmembrane domain (aa 819-839), and the cytoplasmic domain (aa 840-1032). It is expressed in the basolateral region of gastric epithelial cells and high levels are detected in antrum and body mucosa. Ligand recognition of TLR9 is shown to be strictly controlled to prevent unnecessary response to self-derived DNA such as mitochondrial DNA. Ligand-dependent TLR9 trafficking plays a critical role in type I interferon induction in macrophages and plasmacytoid dendritic cells. TLR9 is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response. (Ref.: Murakami, Y., et al. (2017). Sci. Rep. 7; 44042).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2a in PBS without azide.
Applications
Application
Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.
Key Applications
Flow Cytometry
Immunocytochemistry
Inhibits Activity/Function
Application Notes
Immunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
Biological Information
Immunogen
Ba/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.
Clone
NaR9
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.
Evaluated by Immunocytochemistry in Raw264.7 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Toll-like Receptor 9 (TLR9) is an innate immune receptor recognizing microbial DNA. TLR9 is also activated by self-derived DNA, such as mitochondrial DNA, in a variety of inflammatory diseases. We show here that TLR9 activation in vivo is controlled by an anti-TLR9 monoclonal Ab (mAb). A newly established mAb, named NaR9, clearly detects endogenous TLR9 expressed in primary immune cells. The mAb inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. Furthermore, NaR9 treatment rescued mice from fulminant hepatitis caused by administering the TLR9 ligand CpGB and D-(+)-galactosamine. The production of proinflammatory cytokines induced by CpGB and D-(+)-galactosamine was significantly impaired by the mAb. These results suggest that a mAb is a promising tool for therapeutic intervention in TLR9-dependent inflammatory diseases.
