Expression of class 3 semaphorins and their receptors in human breast neoplasia.
Carolyn A Staton,Lucy A Shaw,Manoj Valluru,Leslie Hoh,Ivan Koay,Simon S Cross,Malcolm W Reed,Nicola J Brown
Histopathology
59
2010
Abstract anzeigen
This study aimed to identify the involvement of class 3 semaphorins (Sema3) and receptors, neuropilins (Np1 and Np2) and plexins (A1-A4) in breast cancer development and angiogenesis. | | | 21884206
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Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
Wu, F; Zhou, Q; Yang, J; Duan, GJ; Ou, JJ; Zhang, R; Pan, F; Peng, QP; Tan, H; Ping, YF; Cui, YH; Qian, C; Yan, XC; Bian, XW
Clinical cancer research : an official journal of the American Association for Cancer Research
17
2702-11
2010
Abstract anzeigen
To elucidate the role of Semaphorin-3F (SEMA3F), originally described as an axon guiding chemorepulsant implicated in nerve development, in the progression of colorectal carcinoma.SEMA3F and its receptor NRP2 were examined in 72 cases of human colorectal carcinoma specimens and cell lines LoVo, SW480, and SW620 with immunohistochemistry and Western blotting. SEMA3F mRNA expression in the frozen tissue specimens and cell lines was examined with quantitative reverse transcriptase-PCR. Confocal laser scanning microscopy was used for detection of cellular localization of the proteins by immunofluorescent staining. MTT assay, flow cytometry, cell adhesion and migration, and xenografts were used to evaluate biological significance of SEMA3F.SEMA3F was significantly reduced in colorectal carcinoma tissues and cell lines. Overexpression of SEMA3F resulted in reduced proliferation, adhesion to fibronectin, and migratory capability as well as reduced S-phase population and integrin αvβ3 expression of SW480 colon cancer cells. In addition, SEMA3F-overexpressing cells exhibited diminished tumorigenesis when transplanted orthotopically in nude mice and reduced liver metastases. Moreover, transfection of siRNA targeting SEMA3F in colon cancer cells increased their tumorigenicity in vivo.Endogenous SEMA3F acts as a suppressor of the growth and metastasis of human colorectal cancer cells. | | | 21349996
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Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
Alexander Fassold,Werner Falk,Sven Anders,Thomas Hirsch,Vladimir M Mirsky,Rainer H Straub
Arthritis and rheumatism
60
2009
Abstract anzeigen
In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of this study was to investigate the role of neuropilin-2 in the synovial tissue of patients with RA and patients with osteoarthritis (OA) and in experimental arthritis. | | | 19790074
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