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MABD89
Sigma-AldrichAnti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84
Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry.
More>>Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry. Less<<
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Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84
Alternate Names
Podocalyxin-like protein I
GCTM-2 antigen
Gp200
Background Information
Podocalyxin-like protein 1 (PODXL) is a transmembrane protein known to be heavily sialyated and sulfated that is localized on the apical surface of podocytes and on vascular endothelia. Podocalyxin protein is expressed on the surface of undifferentiated human embryonic stem cells. Originally, the protein was characterized as a marker for vasculature and podocytes, but expression has since been observed in platelets, hematopoietic precursor cells and mesothelial cells from the coelmic cavity lining. Studies have outlined two distinct and seemingly adverse roles for this protein; it acts as an anti-adhesion molecule in renal glomerulae podocytes, but functions as an adhesion molecule within vasculature on high endothelial venules. Podocalyxin expression has been implicated in the development of aggressive cancers, beta catenin signaling, angiogenisis, and both myeloid and acute lymphoblastic leukemias.
References
Product Information
Format
Purified
Control
H9 human embryonic stem cells.
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS without preservatives.
Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry.
Key Applications
Western Blotting
Immunocytochemistry
Flow Cytometry
Application Notes
This antibody was shown to induce cell death of undifferentiated, but not differentiated hESC, within 30 minutes of incubation (Choo, A., et al. (2008). Stem Cells 26:1454-1463). This antibody has been shown to work in Western Blot applications (Choo, A., et al. (2008). Stem Cells, 26:1454–1463). This antibody has been shown to work in Flow Cytometry applications (Choo, AB., et al. (2008). Stem Cells, 26:1454–1463).
Biological Information
Immunogen
HES-3 Human embryonic stem cell line
Clone
mab 84
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the extracellular domain of Podocalyxin-like protein 1. This antibody has been shown to select against undifferentiated human embryonic stem cells (hESC) and to be functionally cytotoxic against these undifferentiated hESC populations (Choo, A., et al., 2008).
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Podocalyxin-like protein I in H9 human embryonic stem cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
MABD89
04053252822551
Documentation
Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 SDB
Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1. Choo, Andre B, et al. Stem Cells, 26: 1454-63 (2008)
2008
Future therapeutic applications of differentiated human embryonic stem cells (hESC) carry a risk of teratoma formation by contaminating undifferentiated hESC. We generated 10 monoclonal antibodies (mAbs) against surface antigens of undifferentiated hESC, showing strong reactivity against undifferentiated, but not differentiated hESC. The mAbs did not cross react with mouse fibroblasts and showed weak to no reactivity against human embryonal carcinoma cells. Notably, one antibody (mAb 84) is cytotoxic to undifferentiated hESC and NCCIT cells in a concentration-dependent, complement-independent manner. mAb 84 induced cell death of undifferentiated, but not differentiated hESC within 30 minutes of incubation, and immunoprecipitation of the mAb-antigen complex revealed that the antigen is podocalyxin-like protein-1. Importantly, we observed absence of tumor formation when hESC and NCCIT cells were treated with mAb 84 prior to transplantation into severe combined immunodeficiency mice. Our data indicate that mAb 84 may be useful in eliminating residual hESC from differentiated cells populations for clinical applications. Disclosure of potential conflicts of interest is found at the end of this article.
