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MABS195 Anti-Pan-Ras Antibody, clone RAS 10

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MABS195
100 µg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS195
      DescriptionAnti-Pan-Ras Antibody, clone RAS 10
      Alternate Names
      • GTPase Kras
      • K-Ras 2
      • Ki-Ras
      • c-K-ras
      • c-Ki-ras
      • GTPase Kras, N-terminally processed
      Background InformationThe human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
      References
      Product Information
      FormatPurified
      Control
      • MCF7 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Pan-Ras.
      CloneRAS 10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRAS
      • KRAS2
      • RASK2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~21 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF7 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS195 04053252440458

      Documentation

      Anti-Pan-Ras Antibody, clone RAS 10 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Pan-Ras Antibody, clone RAS 10 Analysenzertifikate

      TitelChargennummer
      Anti-Pan-Ras, clone RAS 10 2480954
      Anti-Pan-Ras, clone RAS 10 - 2438646 2438646
      Anti-Pan-Ras, clone RAS 10 - 2272141 2272141
      Anti-Pan-Ras, clone RAS 10 - 2294377 2294377
      Anti-Pan-Ras, clone RAS 10 - 2377187 2377187
      Anti-Pan-Ras, clone RAS 10 - 2483437 2483437
      Anti-Pan-Ras, clone RAS 10 - 3180883 3180883
      Anti-Pan-Ras, clone RAS 10 - 3280271 3280271
      Anti-Pan-Ras, clone RAS 10 - 3589763 3589763
      Anti-Pan-Ras, clone RAS 10 - 3731677 3731677

      Literatur

      ÜbersichtPub Med ID
      Production and characterization of anti-RAS p21 monoclonal antibodies.
      Hamer, P J, et al.
      Hybridoma, 9: 573-87 (1990)  1990

      Abstract anzeigen
      2076896 2076896

      Broschüre

      Titel
      New Products: Volume 3, 2012

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies