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07-1216 Anti-PP2A Antibody, Catalytic subunit

This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.

Anti-PP2A Antibody, Catalytic subunit: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: PPP2R5D, MGC8949, MGC2134, OTTHUMP00000039821, B56D UniProt Number: Q14738
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07-1216
100 µL  
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      Übersicht

      Replacement Information
      Replacement Information07-1216 is a recommended replacement for AB1621.

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1216
      ReplacesAB1621
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP2A Antibody, Catalytic subunit
      Alternate Names
      • Serine/threonine protein phosphatase 2A
      • 56 kDa regulatory subunit, delta isoform
      • Delta isoform of regulatory subunit B56, protein phosphatase
      • Protein phosphatase 2, regulatory subunit B (B56), delta isoform
      • Protein phosphatase 2, regulatory subunit B', delta isoform
      Background InformationPP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing PBS with no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.
      Biological Information
      ImmunogenSynthetic peptide corresponding to a.a. 298-309 of the human sequence.
      EpitopeC-terminus
      HostRabbit
      SpecificityRecognizes protein phosphatase 2A, catalytic subunit.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      Species Reactivity NotePeptide is highly conserved in human, rat, bovine, mouse and chicken.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • PPP2R5D
      • MGC8949
      • MGC2134
      • OTTHUMP00000039821
      • B56D
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14738 # The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
      SIZE: 602 amino acids; 69992 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nuclear in interphase, nuclear during mitosis.
      TISSUE SPECIFICITY: Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: Q14738 ## Belongs to the phosphatase 2A regulatory subunit B56 family.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HEK293 lysates.
      Western Blot Analysis:
      1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      07-1216 04053252403989

      Documentation

      Anti-PP2A Antibody, Catalytic subunit SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-PP2A Antibody, Catalytic subunit Analysenzertifikate

      TitelChargennummer
      Anti-PP2A, Catalytic subunit - 2373516 2373516
      Anti-PP2A, Catalytic subunit - 4017407 4017407
      Anti-PP2A, Catalytic subunit - JBC1787314 JBC1787314
      Anti-PP2A, Catalytic subunit - JH1752511 JH1752511
      Anti-PP2A, Catalytic subunit - NG1602923 NG1602923
      Anti-PP2A, Catalytic subunit - NG1800823 NG1800823
      Anti-PP2A, Catalytic subunit Polyclonal Antibody 2898377
      Anti-PP2A, Catalytic subunit Polyclonal Antibody 3111210

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies