Millipore Sigma Vibrant Logo

06-222 Anti-PP2A Antibody, C subunit

View Products on Sigmaaldrich.com
06-222
200 µg  
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
Bitte erfragen
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbIP, WBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-222
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP2A Antibody, C subunit
      Product Information
      FormatSerum
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Other controls: HeLa whole cel lysate, mouse NIH3T3 cells
      PresentationImmunoaffinity Purified immunoglobulin in 150mM Tris, pH 7.4, 0.02% sodium azide, 50% glycerol and 5mg/ml BSA
      Quality LevelMQ100
      Applications
      ApplicationAnti-PP2A Antibody, C subunit is an antibody against PP2A for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to the C-terminal amino acid residues 296-309 of human protein phosphatase 2A
      HostRabbit
      SpecificityRecognizes PP2A-subunit alpha and PP2A-subunit beta; does not react with PP1 or other protein phosphatases.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • RP-C
      • PP2Ac
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit; Binds 1 manganese ion per subunit
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct.
      SUBCELLULAR LOCATION:Cytoplasm.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight36 kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a rabbit brain cytosol or in a human A431 RIPA cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Bestellnummer GTIN
      06-222 04053252675799