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06-1330 Anti-PBX1 Antibody

Anti-PBX1 Antibody is a rabbit polyclonal antibody for detection of PBX1 also known as pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, Homeobox protein PRL & has been validated in WB, ICC.

Anti-PBX1 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function, Stem Cell Research Research Sub-Category: Transcription Factors, Transcription Factors Gene Symbol: PBX1, PRL UniProt Number: P40424
06-1330
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Chp, OpWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1330
      DescriptionAnti-PBX1 Antibody
      Alternate Names
      • pre-B-cell leukemia homeobox 1
      • pre-B-cell leukemia transcription factor 1
      • Homeobox protein PRL
      • Pre-B cell leukemia transcription factor-1
      • Homeobox protein PBX1
      Background InformationPre-B-cell leukemia transcription factor 1 (PBX1) is a TALE-class homeodomain transcription factor belonging to the TALE/PBX homeobox family. PBX1 functions as a transcriptional activator of PF4 in complex with MEIS1, facilitating the formation of trimeric transcriptional complexes with Hox proteins. Expression has been observed in human fetal brain which may play a role in the generation of the olfactory bulb interneurons and in mammalian neurogenesis. Mutations in PBX1 have been associated with pre-B-cell acute lymphoblastic leukemia (B-ALL).
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PBX1 Antibody is a rabbit polyclonal antibody for detection of PBX1 also known as pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, Homeobox protein PRL & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot (SNAP i.d.) Analysis: A 1:500 dilution from a representative lot detected PBX1 on 10 µg of HeLa nuclear extract.

      Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PBX1 in NIH/3T3 and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human PBX1.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PBX1 at the N-terminus.
      Species Reactivity
      • Human
      • Chimpanzee
      • Opossum
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with chimpanzee, opossum, panda, orangutan, platypus, and canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PBX1
      • PRL
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Ref.10

      SUBUNIT STRUCTURE: Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1.

      SUBCELLULAR LOCATION: Nucleus Probable.

      TISSUE SPECIFICTY: Expressed in all tissues except in cells of the B and T lineage.

      INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.

      SEQUENCE SIMILARITIES: Belongs to the TALE/PBX homeobox family.

      Contains 1 homeobox DNA-binding domain.

      SEQUENCE CAUTION: The sequence AAA36764.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~39 and ~53 kDa observed. Due to alternative splicing PBX1 is observed with two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1:2000 dilution of this antibody detected PBX1 on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      06-1330 04053252748547