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AM80 Anti-PAR (Ab-1) Mouse mAb (10H)

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Anti-PAR (Ab-1) Mouse mAb (10H): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Poly (ADP-Ribose)

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AM80
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Dr, H, M, RMMonoclonal Antibody

Preis & Verfügbarkeit

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AM80-100UG
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      		 Kst.-Ampulle 100 μg
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      Description
      OverviewRecognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
      Catalogue NumberAM80
      Brand Family Calbiochem®
      SynonymsAnti-Poly (ADP-Ribose)
      References
      ReferencesKawamitsu, H., et al. 1984. Biochemistry 23, 3771.
      Product Information
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
      Negative controlNormal mouse IgG
      Positive controlEtoposide-treated HL-60 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (2.5 µg/ml)
      Immunocytochemistry (5-20 µg/ml)
      Application CommentsPAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
      Biological Information
      Immunogenpurified poly(ADP-ribose)
      ImmunogenBovine
      Clone10H
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₃
      Species Reactivity
      • Drosophila
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AM80-100UG 04055977228052

      Documentation

      Anti-PAR (Ab-1) Mouse mAb (10H) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-PAR (Ab-1) Mouse mAb (10H) Analysenzertifikate

      TitelChargennummer
      AM80

      Literatur

      Übersicht
      Kawamitsu, H., et al. 1984. Biochemistry 23, 3771.

      Broschüre

      Titel
      Caspases and other Apoptosis Related Tools Brochure
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      SynonymsAnti-Poly (ADP-Ribose)
      ApplicationImmunoblotting (2.5 µg/ml)
      Immunocytochemistry (5-20 µg/ml)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
      BackgroundThis monoclonal antibody is directed against poly(ADP)-ribose (PAR) which is synthesized by the nuclear DNA repair enzyme poly(ADP)-ribose polymerase (PARP). PARP is selectively activated when DNA strands break and catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, such as PARP. The amount of PAR formed in living cells with DNA damage is directly related to the extent of the damage. When DNA is damaged, PAR undergoes a rapid turnover, with a half-life only minutes long, as it is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP)-ribose glycohydrolase (PARG). Upon DNA damage by γ-irradiation or oxidative stress PAR fluorescence can be detected for approximately 10 min. PARP is strongly activated during the so-called execution phase of apoptosis, causing a second round of PAR synthesis. PAR synthesized early in apoptosis is remarkably stable and its immunofluorescence correlates well with other markers of apoptosis.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified poly(ADP-ribose)
      Clone10H
      IsotypeIgG₃
      SpeciesDrosophila, human, mouse, rat
      Positive controlEtoposide-treated HL-60 cells
      Negative controlNormal mouse IgG
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Preservative≤0.1% sodium azide
      CommentsPAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKawamitsu, H., et al. 1984. Biochemistry 23, 3771.