FCMAB113A4 Sigma-AldrichAnti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
More>> Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC. Less<<SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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Übersicht
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Key Spec Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
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| H | FC, ICC | M | AlexaFluor®488 | Monoclonal Antibody |
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| Product Information | |
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| Format | AlexaFluor®488 |
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| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Biological Information | |
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| Immunogen | Recombinant GST fusion protein, human Oct-4 (aa 1-134). |
| Clone | 10H11.2 |
| Host | Mouse |
| Specificity | Reacts with human Oct-4. |
| Isotype | IgG1 |
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| Antibody Type | Monoclonal Antibody |
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| UniProt Number | |
| UniProt Summary | FUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity. SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2. SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern. TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. INDUCTION: Transcriptional activity is positively regulated by PKM2. DOMAIN: The POU-specific domain mediates interaction with PKM2. PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity. MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis. SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain. |
| Molecular Weight | ~44 kDa |
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| Quality Assurance | Evaluated by Flow Cytometry in 2102Ep Cells. |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt |
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| Material Size | 100 tests |
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| Specifications |
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| Global Trade Item Number | |
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| Bestellnummer | GTIN |
| FCMAB113A4 | 04053252623707 |
Documentation
Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate SDB
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Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate Analysenzertifikate
| Titel | Chargennummer |
|---|---|
| Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1741716 | NG1741716 |
| Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NRG1675588 | NRG1675588 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1970353 | 1970353 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1990429 | 1990429 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2063186 | 2063186 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2110542 | 2110542 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2196060 | 2196060 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2227191 | 2227191 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1800880 | NG1800880 |
| Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1832963 | NG1832963 |
Literatur
| Übersicht | Pub Med ID |
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| Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G Nature protocols 7 2029-40 2011 Abstract anzeigen | 23099485
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Verwandte Produkte & Anwendungen
Produktfamilien
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Verwandte Produkte nach: Brand Facete
| Milli-Mark™ |
Kategorien
| Life Science Research > Antibodies and Assays > Primary Antibodies |