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AB16301 Anti-Nitric Oxide Synthase III Antibody

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AB16301
100 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB16301
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase III Antibody
      Alternate Names
      • NOS III
      • eNOS
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nitric Oxide Synthase III Antibody detects level of Nitric Oxide Synthase III & has been published & validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot: 5 μg/mL using chemiluminescent detection. Detects ~135-140kDa band in endothelial cell preparations; Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 5-10 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 596-610 of human endothelial NOS, coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReactive with human NOS-III (eNOS). This immunogen sequence is 100% homologous in human, bovine, rat, and mouse, but reactivity has not been tested.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • eNOS
      • cNOS
      • ECNOS
      • EC-NOS
      • NOSIII
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      AB16301 04053252737374