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MAB5520-I-25UL Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1

Anti-NG2 Chondroitin Sulfate Proteogycan, clone 7.1, Cat. No. MAB5520-I, is a mouse monoclonal antibody that detects NG2 Chondroitin sulfate proteoglycan and is tested for use in Flow Cytometry and Immunoprecipitation.

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MAB5520-I-25UL
25 µL  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberMAB5520-I-25UL
      DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1
      Alternate Names
      • Chondroitin sulfate proteoglycan 4
      • Chondroitin sulfate proteoglycan NG2
      • Melanoma chondroitin sulfate proteoglycan
      • Melanoma-associated chondroitin sulfate proteoglycan
      Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q6UVK1; also known as Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan) is encoded by the CSPG4 (also known as MCSP) gene (Gene ID: 1464) in human. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form that contains a large extracellular domain (aa 30-2224), a transmembrane domain (aa 2225-2245), and a cytoplasmic domain (aa 2246-2322). NG2 plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NG2 Chondroitin Sulfate Proteogycan, clone 7.1, Cat. No. MAB5520-I, is a mouse monoclonal antibody that detects NG2 Chondroitin sulfate proteoglycan and is tested for use in Flow Cytometry and Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated NG2 Chondroitin Sulfate Proteoglycan in Immunoprecipitation applications (Smith, F.O., et al. (1996). Blood;87(3):1123-33).

      Flow Cytometry Analysis: A representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Flow Cytometry applications (Morozov, A., et al. (2010). Clin Cancer Res.;16(23):5630-40; Behm, F.G., et al. (1996). Blood;87(3):1134-9; Smith, F.O., et al. (1996). Blood;87(3):1123-33).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHuman bone marrow stromal cells infected with SV-40.
      EpitopeExtracellular domain
      Clone7.1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 7.1 is a mouse monoclonal antibody that detects NG2 Chondroitin sulfate proteoglycan.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CSPG4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight250.54 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in A375 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in one million A375 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MAB5520-I-25UL 04065266791389