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MABE928 Anti-N-methylpurine-DNA glycosylase Antibody, clone 9F7.1

Anti-N-methylpurine-DNA glycosylase Antibody, clone 9F7.1 is an antibody against N-methylpurine-DNA glycosylase for use in Western Blotting and Immunocytochemistry.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: MPG, AAG, ANPG, MID1 UniProt Number: P29372
MABE928
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABE928
      DescriptionAnti-N-methylpurine-DNA glycosylase Antibody, clone 9F7.1
      Alternate Names
      • N-methylpurine-DNA glycosylase
      • DNA-3-methyladenine glycosylase
      • 3-alkyladenine DNA glycosylase
      • 3-methyladenine DNA glycosidase
      • ADPG
      • N-methylpurine-DNA glycosylase
      Background InformationN-methylpurine-DNA glycosylase (also known as 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, DNA-3-methyladenine glycosylase) is encoded by the MPG (also called AAG, ANPG, MID1) gene in human (NCBI Gene ID 4350). MPG/AAG protein is involved in repair of DNA damage due to alkylation both in the nucleus and mitochondria. Specifically, MPG/AAG protein catalyzes the hydrolysis of the N-glycosyl bond between the alkylated base and deoxyribose, thereby removing 3-methyladenine and 7-methylguanine to form "abasic sites" in the damaged DNA. MPG/AAG protein is also found to exhibit DNA-O-glycosidase activity. MPG/AAG protein is reported to remain associated with the abasic DNA after base removal and, in the presence of small alcohols, catalyze the formation of an O-glycosidic linkage between the alcohol hydroxyl group and C1' of the abasic deoxyribose. Unpon alcohol removal, MPG/AAG protein is shown to catalyze the reverse reaction by hydrolyzing the O-glycosidic bonds of alcohol-DNA adducts. (PMID 23290262 & 23688261). Clone 9F7.1 detects an epitope within human MPG/AAG protein (UniProt P29372) N-terminal region, which is not well conserved in murine protein (UniProt Q04841).
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG1κ cell culture supernatant without preservatives.
      Quality LevelMQ300
      Applications
      ApplicationAnti-N-methylpurine-DNA glycosylase Antibody, clone 9F7.1 is an antibody against N-methylpurine-DNA glycosylase for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:20 dilution from a representative lot detected N-methylpurine-DNA glycosylase in HeLa and A431 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human N-methylpurine-DNA glycosylase near the N-terminus.
      EpitopeNear N-terminus
      Clone9F7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MPG
      • AAG
      • ANPG
      • MID1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~37 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected N-methylpurine-DNA glycosylase in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE928 04055977162813