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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
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Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.
More>>Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting. Less<<
Cadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. Its expression is observed in cells of the nervous tissue, vascular smooth muscle cells, and myocytes. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-25) and a propeptide (aa 26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. A 69 amino acid segment of EC-4 of N-cadherin is reported to be essential and sufficient to increase cell motility and promote epithelial to mesenchymal transition. (Ref.: Kim, JB., et al. (2000). J. Cell Biol. 151(6); 1193-1205; Navarro, P., et al. (1998). J. Cell Biol. 140(6); 1475-1484).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Immunocytochemistry
Flow Cytometry
Inhibition
Application Notes
Tested applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected N-Cadherin in Human brain and cerebellum tissue extracts.
Western Blotting Analysis: A representative lot detected N-Cadherin in Western Blotting applications (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).
Flow Cytometry Analysis: 1 µg from a representative lot detected N-Cadherin in one million KG-1 cells.
Inhibition Assay: A representative lot inhibited motility of BT20 breast cancer cells (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).
Flow Cytometry Analysis: A representative lot detected N-Cadherin in Flow Cytometry applications (Wahlig, S., et al. (2020). Sci Rep. 10(1):1681).
Immunocytochemistry Analysis: A representative lot detected Cadherin-2 in Immunocytochemistry applications (Navarro, P., et al. (1998). J Cell Biol.;140(6):1475-1484).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
MBP-tagged recombinant fragment corresponding to 502 amino acids from the extracellular domain of human N-Cadherin.
Epitope
Extracellular domain
Clone
8C11
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 8C11 is a mouse monoclonal antibody that detects N-Cadherin. It targets an epitope within the extracellular domain.
~105 and 130 kDa observed; 99.81 and 97.04 kDa, respectively for isoforms 1 and 2. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Human heart tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-2 in Human heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
