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MABS1171 Anti-MPST Antibody, clone 1H10.1

This Anti-MPST Antibody, clone 1H10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of MPST.

Anti-MPST Antibody, clone 1H10.1 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: Toxicology & Drug Resistance Gene Symbol: MPST, TST2 UniProt Number: P25325
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MABS1171
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1171
      DescriptionAnti-MPST Antibody, clone 1H10.1
      Alternate Names
      • 3-mercaptopyruvate sulfurtransferase
      • MpST
      • MST
      • tRNA thiouridine modification protein
      • TUM1
      Background Information3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2; UniProt P25325; also known as MpST, MST, tRNA thiouridine modification protein, TUM1) is encoded by the MPST (also known as TST2) gene (Gene ID 4357) in human. 3-mercaptopyruvate sulfurtransferase (MpST) catalyzes the transfer of a sulfur ion to cyanide or other thiol compounds and plays a central role in both cysteine degradation and cyanide detoxification. MpST is also known as tRNA thiouridine modification protein (TUM1) and is implicated in other cellular processes in addition to cyanide detoxification, ranging from thiolation of cytosolic tRNAs to the generation of H2S as signaling molecule both in mitochondria and the cytosol. Chronic cyanide intoxications caused by cyanogenic substances present in food products can lead to neurological disorders. MpST and rhodanese [EC 2.8.1.1] are two enzymes that catalyze cyanide detoxification, with MpST also displaying weak rhodanese activity. Cyanide detoxification by rhodanese requires the presence of compounds bearing labile sulfane sulfur, while mercaptopyruvate is needed for MpST catalyzed reaction. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate, resulting in an inactivation of MpST enzymatic activity. Reduced thioredoxin reactivates the enzyme by cleaving an intersubunit disulfide bond to turn the redox switch on. Aberrant MpST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MPST Antibody, clone 1H10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of MPST.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected MPST in 10 µg of human liver tissue lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MPST in human heart and kidney tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant fragment from the second Rhodanese domain of human MPST.
      EpitopeSecond Rhodanese domain.
      Clone1H10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1H10.1 targets an epitope in the second Rhodanese domain present in both spliced isoforms of human MPST reported by UniProt (P25325).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MPST
      • TST2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~33 kDa observed. 33.05 kDa (isoform 1) and 35.12 kDa (isoform 2) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SHSY-5Y cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPST in 10 µg of SHSY-5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1171 04055977353006