Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-JAM-A/F11R, clone CSTEM27, Cat. No. MABT1541, is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
More>>Anti-JAM-A/F11R, clone CSTEM27, Cat. No. MABT1541, is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting. Less<<
Junctional adhesion molecule A (UniProt: Q9Y624; also known as JAM-A, Junctional adhesion molecule 1, JAM-1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321) is encoded by the F11R (also known as JAM1, JCAM) gene (Gene ID: 50848) in human. JAM-A/F11R is a single-pass type I membrane protein that is localized at tight junctions of both epithelial and endothelial cells. It is also expressed in circulating platelets and leukocytes. It is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 28-238), a transmembrane domain (aa 239-259), and a cytoplasmic domain (aa 260-299). The extracellular domain contains a membrane distal VH-type Ig-like domain (D1; aa 28-125) and a membrane proximal C2-type Ig-like domain (D2; aa 135-228) that contains a single glycosylation site (Asn 185). The cytoplasmic domain includes two phosphorylation sites (Tyr 280 and Ser 284) and also has a type II PDZ domain-binding motif (aa 297-299). JAM-A/F11R plays a role in epithelial tight junction formation and also regulates monocyte transmigration involved in integrity of epithelial barrier. It participates in the regulation of various biological processes, including paracellular permeability, tight junction formation and maintenance, leukocyte trans-endothelial migration, epithelial-to-mesenchymal transition (EMT), angiogenesis, reovirus binding, and platelet activation. Its dysregulation may contribute to pathological consequences, such as cardiovascular diseases, inflammatory bowel disease, neurological disorders, and various cancer types. In cancer cells, it is not only associated with regulation of cell migration, but it also affects apoptosis, EMT, cancer stem cell maintenance, and cell proliferation. Down-regulation of JAM-A/F11R results in elevated cell migration, invasion, and metastasis in various breast cancer cell lines. Its higher levels have been linked to reduced cell migration ability of cancer cells. (Ref.: Czubak-Prowizor, K., et al. (2022). Mol. Cell. Biochem. 477(1); 79-98; Liu, X., et al. (2020). Nature. 586(7827); 101-107).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-JAM-A/F11R, clone CSTEM27, Cat. No. MABT1541, is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
Western Blotting
Flow Cytometry
ELISA
Immunocytochemistry
Application Notes
Tested Applications
Flow Cytometry Analysis: A representative lot detected JAM-A/F11R in Flow Cytometry application (Liu, X., et al. (2017). Nat Methods. 14(11); 1055-1062; O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640; Liu, X., et al. (2020). Nature. 586(7827); 101-107).
ELISA Analysis: A representative lot detected JAM-A/F11R in ELISA application (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).
Immunocytochemistry Analysis: A representative lot detected JAM-A/F11R in Immunocytochemistry application (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to the extracellular domain of human Junctional adhesion molecule A (JAM-A/F11R).
Epitope
Extracellular domain
Clone
CSTEM27
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone CSTEM27 is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11). It targets an epitope within the extracellular domain.
~32 kDa observed; 32.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting with recombinant Human JAM-A/F11R protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human JAM-A/F11R protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
