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MAB1951Z Anti-Integrin β1 Antibody, clone P4G11, azide free

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MAB1951Z
100 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RbELISA, FC, IP, FUNC, FUNC, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1951Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone P4G11, azide free
      Alternate Names
      • CD29
      • MAB1951
      Product Information
      FormatPurified
      PresentationSterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Affects Function
      • Affects Function
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

      Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

      Traditional formalin fixation is not recommended.

      Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

      Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

      FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

      Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman IL-1beta activated endothelial cells
      CloneP4G11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHuman integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • CD29
      • MSK12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      MAB1951Z 04053252317774