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386023 Anti-Hsp47 Mouse mAb (M16.10A1)

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Anti-Hsp47 Mouse mAb (M16.10A1): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Colligin

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386023
Preis & Verfügbarkeit

Übersicht

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Gp, Ht, H, Mk, M, Porcine, R, ShMMonoclonal Antibody

Preis & Verfügbarkeit

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386023-50UG
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      		 Kst.-Ampulle 50 μg
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      Description
      OverviewRecognizes the ~47 kDa Hsp47 protein.
      Catalogue Number386023
      Brand Family Calbiochem®
      SynonymsAnti-Colligin
      Application Data
      Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence.

      (left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
      References
      ReferencesKuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
      Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
      Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
      Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
      Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
      Saga, S., et al. 1987. J. Cell Biol. 105, 517.
      Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive control3T3 cells, Human breast cancer tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme Immunoassay
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesELISA (see comments)
      Enzyme Immunoassay (see comments)
      Immunoblotting (1 µg/ml, colorimetric)
      Immunocytochemistry (1 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation (5 µg/ml)
      Paraffin Sections (see comments)
      Application CommentsThis antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      ImmunogenHsp47 from L6 cells
      ImmunogenRat
      CloneM16.10A1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Guinea Pig
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Bestellnummer GTIN
      386023-50UG 04055977189872