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MABE1064-25UG Anti-HsSAS-6 Antibody, clone 91.390.21

Detect SASS6 using this mouse monoclonal Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064-25UG, validated for use in Immunofluorescence, Immunoprecipitation and Western Blotting.

Anti-HsSAS-6 Antibody, clone 91.390.21: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Gene Symbol: SASS6, SAS6 UniProt Number: Q6UVJ0
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MABE1064-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1064-25UG
      DescriptionAnti-HsSAS-6 Antibody, clone 91.390.21
      Alternate Names
      • Spindle assembly abnormal protein 6 homolog
      Background InformationSpindle assembly abnormal protein 6 homolog (UniProt: Q6UVJ0; also known as HsSAS-6) is encoded by the SASS6 (also known as SAS6) gene (Gene ID: 163786) in human. HsSAS-6 is a central scaffolding component of the centrioles that ensure their 9-fold symmetry. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. It is required for centrosome biogenesis and duplication and is essential for both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multi-ciliated cells. It contains a 35 nM long coiled-coil domain that mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring. Over-expression of SASS6 gene results in excess foci-bearing centriolar markers. Mutations in SASS6 are known to cause microcephaly 14 that is characterized by small head circumference, markedly reduced brain weight and disproportionately small cerebral cortex.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect SASS6 using this mouse monoclonal Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064-25UG, validated for use in Immunofluorescence, Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected HsSAS-6 in 10 µg of HeLa cell lysate.
      Western Blotting Analysis: A representative lot detected HsSAS-6 in RNAi in U2OS cell lysates (Courtesy of Professor Dr. Erich Nigg, Ph.D; Biozentrum, University of Basel, Switzerland).
      Immunofluorescence Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunofluorescence (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).
      Immunofluorescence Analysis: A representative lot detected HsSAS-6 in Immunofluorescence applications (Kleylein-Sohn, J. et. al. (2007). Dev Cell. 13(2):190-202 ; Sonnen, K..,et. al. (2012). Biol Open 1(10):965-76; Arquint, C., et al. (2012). J Cell Sci. 125(Pt 5):1342-52 ).
      Immunoprecipitation Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunoprecipitation (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 254 amino acids from the C-terminal half of human Spindle assembly abnormal protein 6 homolog (HsSAS-6).
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 91.390.21 detects HsSAS-6 protein in human cells. It targets an epitope with in 254 amino acids in the C-terminal half.
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SASS6
      • SAS6
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U20S cell lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected HsSAS-6 in 10 µg of  U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1064-25UG 04054839437946