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ABE491 Anti-Histone-lysine N-methyltransferase NSD3 Antibody

Detect Histone-lysine N-methyltransferase NSD3 using this rabbit polyclonal antibody, Anti-Histone-lysine N-methyltransferase NSD3 Antibody validated for use in western blotting & IHC.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: WHSC1L1, NSD3, DC28 UniProt Number: Q9BZ95
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ABE491
200 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE491
      DescriptionAnti-Histone-lysine N-methyltransferase NSD3 Antibody
      Alternate Names
      • Histone-lysine N-methyltransferase NSD3
      • Nuclear SET domain-containing protein 3
      • Protein whistle
      • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
      • Wolf-Hirschhorn syndrome candidate 1-like protein 1
      • WHSC1-like protein 1
      Background InformationHistone-lysine N-methyltransferase NSD3, also known as Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine or Wolf-Hirschhorn syndrome candidate 1-like protein 1, (WHSC1-like protein 1), and encoded by the gene WHSC1L1/NSD3, is a histone methylase that preferentially methylates Lys-4 and Lys-27 of histone H3. Lys-4 methylation is generally a specific tag for transcriptional activation while Lys-27 methylation is a mark for transcriptional repression. NSD3 is highly expressed in many tissues including the brain, heart and skeletal muscle. Overexpression of NSD3 is associated with certain forms of lung cancer as well as certain other cancers. NSD3 also facilitates the expression of many other genes important in cancer progression such as Brd4. Brd4 through its extraterminal domain interacts with NSD3 which regulates transcription by modifying the chromatin microenvironment at Brd4 target genes.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Histone-lysine N-methyltransferase NSD3 using this rabbit polyclonal antibody, Anti-Histone-lysine N-methyltransferase NSD3 Antibody validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone-lys N-methyltransferase NSD3 in human and mouse cerebellum tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone-lys N-methyltransferase NSD3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody is expected to cross react with isofomrs 1, 2, and 4 based upon immunogen design.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WHSC1L1
      • NSD3
      • DC28
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~180 kDa, ~160 kDa, ~90 kDa, and ~70 kDa observed. Uniprot desribes four isoforms at 162 kDa (isoform 1), 156 kDa (isoform 2), 73 kDa (isoform 3), and isoform 4 (155 kDa). This protein is known to be acetylated and phosphorylated and may be observed at higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in BALB/3T3 nuclear extract.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Histone-lys N-methyltransferase NSD3 in 10 µg of BALB/3T3 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE491 04053252930850