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MABE979 Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2

This Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2 is validated for use in Western Blotting for the detection of Histone Demethylase JARID1C.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: KDM5C, DXS1272E, JARID1C, MRXSCJ, SMCX, XE169 UniProt Number: P41229
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MABE979
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE979
      DescriptionAnti-Histone Demethylase JARID1C Antibody, clone 2B4.2
      Alternate Names
      • Lysine-specific demethylase 5C
      • Histone demethylase JARID1C
      • Jumonji/ARID domain-containing protein 1C
      • Protein SmcX
      • Protein Xe169
      Background InformationLysine-specific demethylase 5C (UniProt P41229; also known as Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169) is encoded by the KDM5C (also known as DXS1272E, JARID1C, MRXSCJ, SMCX, XE169) gene (Gene ID 8242) in human. RBP2 (JARID1A), PLU-1 (JARID1B), KDM5C (JARID1C), and SMCY (JARID1D) constitute the Jumonji/ARID (JARID) subfamily of JmjC-containing proteins. KDM5C/JARID1C catalyzes the demethylation of di- and trimethylated lysine 4 of histone 3 in a Fe(II) and α-ketoglutarate-dependent manner. JARID1 catalytic activity is medidated by its JmjC domain (a.a. 468-634), while the JmjN domain (a.a. 14-55) and the C5HC2 zinc finger (a.a. 326-372 & 1187-1248) are both important in assisting JARID1C catalytic activity. JARID1C also contains an AT-rich domain-interacting domain (ARID) that binds DNA and its N-terminal PHD (plant homeodomain) finger (a.a. 326-372) is reported to bind trimethylated lysine 9 on histone 3 (H3K9me3). JARID1C interacts with PCNA through its PCNA-interaction protein (PIP) motif and JARID1C is among 485 chromatin factors enriched at nascent versus mature post-replicative chromatin, indicating a potential transcription-independent role during DNA replication.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2 is validated for use in Western Blotting for the detection of Histone Demethylase JARID1C.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an N-terminal region sequence of human JARID1C.
      EpitopeN-terminal region.
      Clone2B4.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2B4.2 targets an N-terminal region sequence present in all human and murine JARID1C/KDM5C spliced isoforms reported by UniProt (P41229 and P41230).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM5C
      • DXS1272E
      • JARID1C
      • MRXSCJ
      • SMCX
      • XE169
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~180 kDa observed. 175.7/175.3 kDa (human/mouse isoform 1), 175.4/175.0 kDa (human/mouse isoform 2), 170.6/170.2 kDa (human/mouse isoform 3), 155.1/175.6 kDa (human isoform 4/5) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected histone demethylase JARID1C in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE979 04055977352887

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies