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Anti-GATA6 Antibody, clone 1G2.2 is an antibody against GATA6 for use in Western Blotting.
More>>Anti-GATA6 Antibody, clone 1G2.2 is an antibody against GATA6 for use in Western Blotting. Less<<
Anti-GATA6 Antibody, clone 1G2.2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
GATA-6 is a developmental transcriptional activator important in terminal differentiation and proliferation of cells destined to be associated with the heart, gut and gut related tissues and organs. GATA-6 works in concert with SEMA3C and PLXNA2 transcription factors and regulates the expression of their target genes. GATA-6 is particularly important in heart development as a number of genetic mutations within GATA-6 give rise to defective syndromes involving the heart or the vessels of the heart. Interestingly, recent research into pulmonary hypertension disease in adults points to a critical role of GATA-6 expression in endothelial cells lining the blood vessels. Healthy blood vessels express GATA-6 very strongly but expression is lost during vascular injury and markedly down regulated in the endothelial cells found in diseased vessels. Because GATA-6 is a direct transcriptional regulator or important vascular proteins such as endothelin-1 receptor, endothelial nitric oxide synthase, and cytokines such as Fractalkine, GATA-6’s role in modulating the effects of pulmonary hypertension and other vascular syndromes is a subject of much research.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Anti-GATA6 Antibody, clone 1G2.2 is an antibody against GATA6 for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GATA6 in 10 µg of human brain, human heart, human lung, human liver, human kidney, human skin, human spleen, human thymus, human pancreas, human ovary, human testis, human small intestine, human parathyroid, and human adrenal tissue lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human GATA6.
~70 and 40 kDa observed. The calculated molecular weight is 60 kDa, however GATA6 has been shown as a ~70 and ~40 kDa band in some western blots (Van Berlo, J.H., et al. (2010). Cir. Res. 107:1032-1040). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human adrenal, human pancreas, human small intestinetissue, and human heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GATA6 in 10 µg of human adrenal, human pancreas, human small intestinetissue, and human heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Circulation research van Berlo, JH; Elrod, JW; van den Hoogenhof, MM; York, AJ; Aronow, BJ; Duncan, SA; Molkentin, JD Circulation research
107
1032-40
2009
The transcriptional code that programs maladaptive cardiac hypertrophy involves the zinc finger-containing DNA binding factor GATA-4. The highly related transcription factor GATA-6 is also expressed in the adult heart, although its role in controlling the hypertrophic program is unknown.To determine the role of GATA-6 in cardiac hypertrophy and homeostasis.Here, we performed a cardiomyocyte-specific conditional gene targeting approach for Gata6, as well as a transgenic approach to overexpress GATA-6 in the mouse heart. Deletion of Gata6-loxP with Nkx2.5-cre produced late embryonic lethality with heart defects, whereas deletion with β-myosin heavy chain-cre (βMHC-cre) produced viable adults with >95% loss of GATA-6 protein in the heart. These latter mice were subjected to pressure overload-induced hypertrophy for 2 and 6 weeks, which showed a significant reduction in cardiac hypertrophy similar to that observed Gata4 heart-specific deleted mice. Gata6-deleted mice subjected to pressure overload also developed heart failure, whereas control mice maintained proper cardiac function. Gata6-deleted mice also developed less cardiac hypertrophy following 2 weeks of angiotensin II/phenylephrine infusion. Controlled GATA-6 overexpression in the heart induced hypertrophy with aging and predisposed to greater hypertrophy with pressure overload stimulation. Combinatorial deletion of Gata4 and Gata6 from the adult heart resulted in dilated cardiomyopathy and lethality by 16 weeks of age. Mechanistically, deletion of Gata6 from the heart resulted in fundamental changes in the levels of key regulatory genes and myocyte differentiation-specific genes.These results indicate that GATA-6 is both necessary and sufficient for regulating the cardiac hypertrophic response and differentiated gene expression, both alone and in coordination with GATA-4.
