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04-1005 Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: FKH1 , FKHR, FOXO1A, OTTHUMP00000018301 UniProt Number: Q12778
04-1005
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IH(P)RbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1005
      DescriptionAnti-FKHR Antibody, clone EP927Y, rabbit monoclonal
      Alternate Names
      • Forkhead box protein O1A
      • Forkhead in rhabdomyosarcoma
      • forkhead box O1
      • forkhead box O1A (rhabdomyosarcoma)
      • forkhead homolog in rhabdomyosarcoma
      Background InformationThe Non-Radioactive FoxO1 Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the consensus sequence for FoxO1 binding (5'-TTGTTTAC-3'), is mixed with whole cell or nuclear extract in the Transcription Factor Assay Buffer provided directly in the Streptavidin coated plate. When incubated together, FoxO1 contained in the nuclear extract binds to its consensus sequence. The biotinylated double stranded oligonucleotide bound to FoxO1 protein is immobilized and any inactive, unbound material is washed away. The bound FoxO1 transcription factor is detected with a specific rabbit anti-FoxO1, primary antibody. An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader. Included in the kit are positive control whole cell extract, a non-specific double stranded oligonucleotide, and a specific double stranded competitor oligonucleotide.
      References
      Product Information
      FormatUnpurified
      Control
      • NIH/3T3 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected FKHR in human bladder carcinoma tissue.
      Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected FKHR in HeLa cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding Ser329 of human FKHR.
      EpitopeSer329
      CloneEP927Y
      HostRabbit
      SpecificityThis antibody recognizes both phosphorylated and unphosphorylated FKHR.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FKH1
      • FKHR
      • FOXO1A
      • OTTHUMP00000018301
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor. Ref.9
      SUBUNIT STRUCTURE: Interacts with LRPPRC By similarity.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
      SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.
      Molecular Weight~80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on NIH/3T3 cell lysates.
      Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect FKHR in NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      04-1005 04053252619175

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies