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MABE977 Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody

This Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of E3 ubiquitin-protein ligase UHRF1.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Uhrf1, Np95 UniProt Number: Q8VDF2
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MABE977
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE977
      DescriptionAnti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody
      Alternate Names
      • E3 ubiquitin-protein ligase UHRF1
      • Nuclear protein 95
      • Nuclear zinc finger protein Np95
      • Ubiquitin-like PHD and RING finger domain-containing protein 1
      • mUhrf1
      • Ubiquitin-like-containing PHD and RING finger domains protein 1
      Background InformationUbiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of E3 ubiquitin-protein ligase UHRF1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in NIH/3T3 cells.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in 5 mg of NIH/3T3 cell lysate (Roberto Papait, FIRC Institute of Molecolar Oncology).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to mouse E3 ubiquitin-protein ligase UHRF1.
      CloneBF18.11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Uhrf1
      • Np95
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~88 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH-3T3 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of NIH-3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE977 04053252984846