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AB1536 Anti-Dopamine β Hydroxylase Antibody, CT

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AB1536
100 µL  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, R, RbIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1536
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Dopamine β Hydroxylase Antibody, CT
      Alternate Names
      • DBH
      Product Information
      FormatAffinity Purified
      PresentationLiquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Dopamine β Hydroxylase Antibody, C-terminus is an antibody against Dopamine β Hydroxylase for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunoblotting: 1:250-1:1,000.

      Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed moderate staining on both human and monkey.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, CPTSQGRSPAGPTVVSI-amide, cloned from human (DbH) cDNA (Nagatsu et al. 1990).
      EpitopeC-terminus
      HostRabbit
      SpecificityDopamine Beta Hydroxylase (DbetaH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.
      Species Reactivity
      • Human
      • Monkey
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
      Gene Symbol
      • DBH
      • DBM
      • EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline.
      COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit.
      SIZE: 617 amino acids; 69065 Da
      SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers.
      SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential).
      DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
      SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Bestellnummer GTIN
      AB1536 04053252329531