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09-454
Sigma-AldrichAnti-Dock2 Antibody
Anti-Dock2, Cat. No. 09-454, is a rabbit polyclonal antibody that detects Dock2 and is tested for use in Immunohistochemistry and Western Blotting.
More>>Anti-Dock2, Cat. No. 09-454, is a rabbit polyclonal antibody that detects Dock2 and is tested for use in Immunohistochemistry and Western Blotting. Less<<
Anti-Dock2 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Dedicator of cytokinesis protein 2 (UniProt: Q8C3J5; also known as Protein Hch, Dock2) is encoded by the Dock2 gene (Gene ID: 94176) in murine species. Dock2 is a homodimeric protein that is predominantly expressed in immune cells and is involved in cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. It is shown to be critical for migration, activation, proliferation, and effects of immune cells, including lymphocytes, neutrophils, macrophages, and dendritic cells. It is shown to activate RAC1 and RAC2, by functioning as a guanine nucleotide exchange factor (GEF) that exchanges bound GDP for free GTP. Dock2 plays an important role in the host immune response to SARS-CoV-2 infection and in the development of severe COVID-19. Its expression is suppressed in subjects with severe cases of COVID-19. (Ref.: Namkoong, H., et al. (2022). Nature. 609(7928); 754-760; Ji, L., et al. (2022). Front. Mol. Biosci. 9; 997659)
References
Product Information
Format
Affinity Purified
Control
Mouse spleen lysate
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Anti-Dock2, Cat. No. 09-454, is a rabbit polyclonal antibody that detects Dock2 and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Dock2 in Human spleen tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Dock2 in Immunohistochemistry applications (Cimino, PJ et al. (2009). Am. J. Pathol. 175(4); 1622-1630).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of mouse Dock2.
Epitope
Internal region
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Dock2. It targets an epitope within 14 amino acids from the C-terminal region.
Isotype
IgG
Species Reactivity
Mouse
Human
Species Reactivity Note
Human, Mouse. Predicted to react with Rat, Horse, Non-Human Primates based on 100% sequence homology.
FUNCTION: Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May participate in IL2 transcriptional activation via the activation of RAC2. Ref.1
SUBUNIT: Interacts with RAC1 and RAC2. Interacts with CRKL and VAV. Interacts with CD3Z By similarity.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane proteinBy similarity. Cytoplasm › cytoskeletonBy similarity. Note= Colocalizes with F-actin By similarity.
TISSUE SPECIFICITY: Specifically expressed in hematopoietic cells. Ref.1
DOMAIN: The DHR-2 domain probably mediates the GEF activity By similarity.
SIMILARITY: Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain.
Molecular Weight
~180 kDa observed; 211.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Mouse Spleen tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Dock2 in Mouse spleen tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Addendum: defective Dock2 expression in a subset of ASC-deficient mouse lines. Sirish K Ippagunta,R K Subbarao Malireddi,Patrick J Shaw,Geoffrey A Neale,Lieselotte Vande Walle,Yoshinori Fukui,Douglas R Green,Mohamed Lamkanfi,Thirumala-Devi Kanneganti Nature immunology
13
2011
DOCK2 is a microglial specific regulator of central nervous system innate immunity found in normal and Alzheimer's disease brain. Cimino, PJ; Sokal, I; Leverenz, J; Fukui, Y; Montine, TJ The American journal of pathology
175
1622-30
2009
Neuroinflammation is a hallmark of several neurodegenerative diseases, including Alzheimer's disease (AD). Strong epidemiological and experimental evidence supports the use of nonsteroidal anti-inflammatory drugs to reduce AD risk. However, poor outcome in clinical trials and toxicity in a prevention trial have shifted focus away from these cyclooxygenase (COX) inhibitors to seek additional therapeutic targets in the prostaglandin pathway. Previously, the prostaglandin E2 receptor, EP2, was shown to regulate neuroinflammation and reduce Abeta plaque burden in transgenic mice. Unfortunately, widespread EP2 distribution and a direct effect on COX2 induction make EP2 a less desirable target. In this study, we link dedicator of cytokinesis 2 (DOCK2) to the prostaglandin pathway in the brain. Additionally, we show that DOCK2 regulates microglial innate immunity independent of COX2 induction and that DOCK2+ microglia are associated with human AD pathology. Together, these results suggest DOCK2 as a COX2 expression-independent therapeutic target for neurodegenerative diseases such as AD.
Differential requirement for DOCK2 in migration of plasmacytoid dendritic cells versus myeloid dendritic cells. Gotoh, Kazuhito, et al. Blood, 111: 2973-6 (2008)
2008
A central role for DOCK2 during interstitial lymphocyte motility and sphingosine-1-phosphate-mediated egress. Nombela-Arrieta, César, et al. J. Exp. Med., 204: 497-510 (2007)
2007
Non-adherent cell-specific expression of DOCK2, a member of the human CDM-family proteins. Nishihara, H, et al. Biochim. Biophys. Acta, 1452: 179-87 (1999)
1998
