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Anti-Dengue Virus 4 NS1, clone 626, Cat. No. MABF2189, is a mouse monoclonal antibody that detects Dengue virus 4 NS1 protein and is tested for use in ELISA and Flow Cytometry.
More>>Anti-Dengue Virus 4 NS1, clone 626, Cat. No. MABF2189, is a mouse monoclonal antibody that detects Dengue virus 4 NS1 protein and is tested for use in ELISA and Flow Cytometry. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2189-25UG
Description
Anti-Dengue Virus 4 NS1 Antibody, clone 626
Alternate Names
DENV 4 NS1 protein
Dengue virus 4 NS1 protein
Background Information
Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. Clone 626 displays high specificity for DENV 4 NS1. (Ref.: Chen, RU et al. (2018). J Biomed Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Dengue Virus 4 NS1, clone 626, Cat. No. MABF2189, is a mouse monoclonal antibody that detects Dengue virus 4 NS1 protein and is tested for use in ELISA and Flow Cytometry.
Key Applications
ELISA
Flow Cytometry
Application Notes
ELISA Analysis: A representative lot detected Dengue Virus 4 NS1 in ELISA applications (Bosch, I., et. al. (2017). Sci Transl Med. 9(409); eaan1589).
Flow Cytometry Analysis: A representative lot detected Dengue Virus 4 NS1 in Flow Cytometry applications (Bosch, I., et. al. (2017). Sci Transl Med. 9(409); eaan1589).
Biological Information
Immunogen
Purified recombinant Dengue virus NS1 protein expressed in mammalian cells.
Clone
626
Host
Mouse
Specificity
Clone 626 is a mouse monoclonal antibody that detects DENV 4 NS1 protein. It targets an epitope within the wing/finger and beta-ladder domains.
~48 kDa calculated for NS1 protein; ~378.38 kDa for the entire genome polyprotein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by ELISA in DENV4 NS1 recombinant protein.
ELISA Analysis: 5 µg/mL of this antibody detected Dengue Virus 4 NS1 recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cytochrome b of human neutrophils is the central component of the microbicidal NADPH-oxidase system. However, the folding topology of this integral membrane protein remains undetermined. Two random-sequence bacteriophage peptide libraries were used to map structural features of cytochrome b by determining the epitopes of monoclonal antibodies (mAbs) 44.1 and 54.1, specific for the p22phox and gp91phox cytochrome b chains, respectively. The unique peptides of phage selected by mAb affinity purification were deduced from the phage DNA sequences. Phage selected by mAb 44.1 displayed the consensus peptide sequence GGPQVXPI, which is nearly identical to 181GGPQVNPI18 of p22phox. Phage selected by mAb 54.1 displayed the consensus sequence PKXAVDGP, which resembles 382PKIAVDGP389 of gp91phox. Western blotting demonstrated specific binding of each mAb to the respective cytochrome b subunit and selected phage peptides. In flow cytometric analysis, mAb 44.1 bound only permeabilized neutrophils, while 54.1 did not bind intact or permeabilized cells. However, mAb 54.1 immunosedimented detergent-solubilized cytochrome b in sucrose gradients. These results suggest the 181GGPQVNPI188 segment of p22phox is accessible on its intracellular surface, but the 382PKIAVDGP389 region on gp91phox is not accessible to antibody, and probably not on the protein surface.
