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This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .
More>>This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex . Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4). Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an appealing topic in molecular biomedical research.
References
Product Information
Format
Purified
HS Code
3002 15 90
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .
Key Applications
Immunocytochemistry
Immunohistochemistry (Paraffin)
ELISA
Flow Cytometry
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA G-quadruplex (G4) in human pancreas and mouse kidney tissue.
Immunocytochemistry Analysis: A representative lot detected discrete DNA G-quadruplex (G4) foci on the compact chromosome in the metaphase HeLa and murine embryonic stem cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected greatly diminished nuclear DNA G-quadruplex (G4) immunoreactivity upon DNase treatment of paraformaldehyde-fixed and Triton X-100-permeablized HeLa cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected G-quadruplex (G4) stabilizing agent telomestatin/TMS treatment-induced nuclear DNA G4 immunoreactivity in chicken DT40 cells lacking the G4-unwinding helicase FANCJ, while the drug enhancing effect was not seen in human FANCJ-expressing DT40 cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of nuclear DNA G-quadruplex (G4) foci in U20S cells following the treatment of the DNA G4-stabilizing agent telomestatin/TMS (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
ELISA Analysis: Target selectivity of a representative lot was determined by competitive ELISA. Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Flow Cytometry Analysis: A representative lot detected enhanced DNA G-quadruplex (G4) immunoreactivity in HeLa cells upon treatment with the DNA G4-stabilizing agent TMPyP4, Cat. No. 613560 (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunohistochemistry Analysis: A representative lot detected nuclear DNA G-quadruplex (G4) immunoreactivity in various paraffin-embedded human tissue sections, including skin, pancreas, testis, placenta, brain, appendix, colon, and kidney (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Biological Information
Immunogen
KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
Clone
1H6
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Isotype
IgG2bκ
Species Reactivity
All
Species Reactivity Note
All Species. Target DNA structure is not species-specific.
Immunocytochemistry Analysis: 2.0 µg/mL of this antibody detected DNA G-quadruplex (G4) in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
It has been proposed that guanine-rich DNA forms four-stranded structures in vivo called G-quadruplexes or G4 DNA. G4 DNA has been implicated in several biological processes, but tools to study G4 DNA structures in cells are limited. Here we report the development of novel murine monoclonal antibodies specific for different G4 DNA structures. We show that one of these antibodies designated 1H6 exhibits strong nuclear staining in most human and murine cells. Staining intensity increased on treatment of cells with agents that stabilize G4 DNA and, strikingly, cells deficient in FANCJ, a G4 DNA-specific helicase, showed stronger nuclear staining than controls. Our data strongly support the existence of G4 DNA structures in mammalian cells and indicate that the abundance of such structures is increased in the absence of FANCJ. We conclude that monoclonal antibody 1H6 is a valuable tool for further studies on the role of G4 DNA in cell and molecular biology.
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 Antibody[MABE1126_IHC DNA (G4) Antibody-ALL].jpg)
