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MABN1593 Anti-DGCR8 Antibody, clone 11C10.1

Anti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.

Anti-DGCR8 Antibody, clone 11C10.1 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: DGCR8, DGCRK6, LP4941, C22orf12 UniProt Number: Q8WYQ5
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MABN1593
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1593
      DescriptionAnti-DGCR8 Antibody, clone 11C10.1
      Alternate Names
      • Microprocessor complex subunit DGCR8
      • DiGeorge syndrome critical region 8
      • Pasha
      Background InformationMicroprocessor complex subunit DGCR8 (UniProt Q8WYQ5; also known as DiGeorge syndrome critical region 8, Pasha) is encoded by the DGCR8 (also known as DGCRK6, LP4941) gene (ORF C22orf12; Gene ID 54487) in human. The DGCR8 gene encodes a double-stranded RNA-binding protein that functions as a non-catalytic subunit of the microprocessor complex which mediates the biogenesis of microRNAs (miRNA) from the primary microRNA (pri-microRNA) transcript. miRNAs are initially transcribed as pri-microRNA with a local stem-loop that is cleaved by the microprocessor complex to produce the precursor microRNA (pre-miRNA). The microprocessor complex is comprised of the RNase Drosha and the RNA-binding protein DGCR8. DGCR8 is reported to be phosphorylated by Erk and Abl, an event that promotes DGCR8 stability and stimulates Drosha recruitment and subsequent substrate pri-microRNAs processing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant fragment from the region between the WW and DRBM 1 domain of human DGCR8.
      EpitopeRegion between WW and DRBM 1 domain.
      Clone11C10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with spliced isoforms 1 and 3, but not isoform 2 of human DGCR8 reported by UniProt (Q8WYQ5).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DGCR8
      • DGCRK6
      • LP4941
      • C22orf12
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~120 kDa observed. 86.05 kDa (isoform 1) and 82.75 kDa (isoform 3). The detected band size is consistent with the reported apparent molecular weight (Ha, M., and Kim, V.N. (2014). Nat. Rev. Mol. Cell Biol. 15(8):509-524).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected DGCR8 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABN1593 04055977331189