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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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ABS1605
Sigma-AldrichAnti-Cytochrome p450 2J Family Antibody
Anti-Cytochrome p450 2J Family Antibody is an antibody against Cytochrome p450 2J Family for use in Western Blotting , Immunohistochemistry (Paraffin).
More>>Anti-Cytochrome p450 2J Family Antibody is an antibody against Cytochrome p450 2J Family for use in Western Blotting , Immunohistochemistry (Paraffin). Less<<
Anti-Cytochrome p450 2J Family Antibody : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Cytochrome P450 2J2 (EC 1.14.14.1; UniProt P51589; also known as Arachidonic acid epoxygenase, CYPIIJ2, Cytochrome P450 subfamily IIJ polypeptide 2, Flavoprotein-linked monooxygenase, Microsomal monooxygenase) is encoded by the CYP2J2 (also known as CPJ2) gene (Gene ID 1573) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum where they function as the terminal enzymes in electron transfer chains. In addition to processing endogenous substrates, CYPs also function to metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consisits of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. The CYP2J subfamily of enzymes are responsible for the epoxidation of endogenous arachidonic acid (AA) into epoxyeicosatrienoic acids (EETs) and 20-hydroxyeicosatetraenoic acid (20-HETE).
Anti-Cytochrome p450 2J Family Antibody is an antibody against Cytochrome p450 2J Family for use in Western Blotting , Immunohistochemistry (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: An 1:8,000 dilution from a representative lot detected cardiomyocytes with overexpressed human CYP2J2 in formalin-fixed, paraffin-embedded heart tissue sections from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific human CYP2J2 expression (Courtesy of Dr. Darryl C. Zeldin, NIEHS, NIH, Research Triangle Park, NC). Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected recombinant CYP2J (2J2, 2J3, 2J5, 2J8, 2J9, 2J12, 2J13), but not CYP2C (2C44, 2C50, 2C54) proteins (Courtesy of Dr. Darryl C. Zeldin, NIEHS, NIH, Research Triangle Park, NC). Western Blotting Analysis: A representative lot detected the overexpressed human CYP2J2 in primary lung endothelial cells (ECs) from transgenic mice carrying human CYP2J2 transgene downstream of the murine Tie2 promoter to allow endothelial expression of human CYP2J2 (Deng, Y., et al. (2011). FASEB J. 25(2):703–713). Western Blotting Analysis: A representative lot detected a 51 kDa CYP2J band in MIH-2 mouse hepatocellular carcinoma cells (HCCs), but not in C26 and MC38 mouse colon cancer cells or T3 mouse small intestine cancer cells (Homma, S., et al. (2009). Clin. Exp. Immunol. 156(2):344-352). Western Blotting Analysis: A representative lot detected different CYP2J banding patterns between prenatal and postnatal human liver microsomal samples from human subjects ranging from 1.1 EGA (estimated gestational age) to 17-year-old of age (Gaedigk, A., et al. (2006). J. Pharmacol. Exp. Ther. 319(2): 523–532). Western Blotting Analysis: A representative lot detected the overexpressed human CYP2J2 in heart tissues from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific transgene expression (Seubert, J., et al. (2004). Circ. Res. 95(5):506-514). Western Blotting Analysis: A representative lot detected a reduced CYP2J level in lung microsome preparations from pneumonia rats when compared with control rats (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105). Western Blotting Analysis: A representative lot detected recombinant human (2J2), rat (2J3 & 2J4), and mouse (2J5, 2J6, 2J8, 2J9) CYP2J proteins (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105). Immunohistochemistry Analysis: A representative lot detected the overexpressed human CYP2J2 in formalin-fixed, paraffin-embedded heart tissue sections from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific transgene expression (Seubert, J., et al. (2004). Circ. Res. 95(5):506-514).
Biological Information
Immunogen
Linear peptide corresponding to an internal sequence from the C-terminal half of human cytochrome p450 2J2.
Epitope
Internal (C-terminal half).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antiserum is also known as Anti-CYP2J2pep3. It cross-reacts with all known CYP2J subfamily P-450s, but not non-CYP2J subfamily P-450s (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105).
Species Reactivity
Rat
Mouse
Human
Species Reactivity Note
Human, Mouse, Rat. Predicted to react with Porcine, Sheep based on 100% sequence homology.
Evaluated by Western Blotting in rat liver tissue lysate.
Western Blotting Analysis: An 1:5,000 dilution of this antibody detected cytochrome p450 2J protein in 10 µg of rat liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
