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This Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free) is validated for use in Western Blotting and Immunocytochemistry and Immunofluorescence and Immunohistochemistry for the detection of Connexin 45, near CT, cytoplasmic.
More>>This Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free) is validated for use in Western Blotting and Immunocytochemistry and Immunofluorescence and Immunohistochemistry for the detection of Connexin 45, near CT, cytoplasmic. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free)
Alternate Names
Gap junction gamma-1 protein
Connexin-45
Gap junction alpha-7 protein
Connexin45
Connexin 45
Background Information
Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.
References
Product Information
Format
Purified
HS Code
3002 15 90
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free) is validated for use in Western Blotting and Immunocytochemistry and Immunofluorescence and Immunohistochemistry for the detection of Connexin 45, near CT, cytoplasmic.
Key Applications
Western Blotting
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot detected Connexin 45 in transfected rat C6 cells.
Immunocytochemistry Analysis: A representative lot detected Connexin 45 in Rat aorta endothelial cells and transfected HeLa cells (Sorensen, C.M., et al. (2008) Am J Physiol Regul Integr Comp Physiol. 295:R840–R847).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in HeLa cells and human cardiomyocytes (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Immunofluorescence Analysis: A representative lot detected Connexin 45 in normal adult rat bladder (Ikeda Y., et al. (2007) Am J Physiol Renal Physiol. 293(4):F1018–F1025).
Western Blotting Analysis: A representative lot detected Connexin 45 in HeLa and human cardiomyocyte lysate (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).
Western Blotting Analysis: A representative lot detected Connexin 45 in guinea pig atrial and SA node tissue (Jones S.A., et al. (2004) The Journal of Physiology, 560:429-437).
Western Blotting Analysis: A representative lot detected Connexin 45 in WT and transfected HeLa cell lysates (Elenes S., et al. (2001) Biophys J. 81(3):1406-18).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in neural tube of 12-day-old embryo and bone marrow spaces and blood vessels after 4 days of orthodontic treatment in periodontium (Gluhak-Heinrich, J., et al. (2006) Cell Commun Adhes. 13(1-2):115–125).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in endothelial and subintimal smooth muscle gap junction plaques in the rabbit iliac artery (Chaytor, A.T., et al. (2005) British Journal of Pharmacology. 144:108–114).
Immunohistochemistry Analysis: A representative lot detected Connexin 45 in rabbit middle cerebral artery (Ujiie H., et al. (2003) Stroke. 34(2):544-50).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to Human Connexin 45.
~45 kDa observed. Additional uncharacterized bands may be visible in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Connexin 45 in 10 µg of U2OS cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clinical studies have shown that sinoatrial node dysfunction occurs at the highest incidence in the elderly population. Guinea-pigs were studied throughout their lifespan (i.e. birth to 38 months) to investigate the possible mechanism leading to nodal dysfunction. Using immunofluorescence with confocal microscopy, Cx43 protein expression was shown at birth to be present throughout the sinoatrial node and atrial muscle, however, at one month Cx43 protein was not expressed in the centre of the sinoatrial node. Throughout the remainder of the animal's lifespan the area of tissue lacking Cx43 protein progressively increased. Western blot provided verification by quantitative analysis that Cx43 protein expression within the sinoatrial node decreased with age; however, the expression of other cardiac connexins, Cx40 and Cx45, did not differ with age. Analysis of conduction maps showing propagation of the action potential across the sinoatrial node, from the initiation point to the crista terminalis, found that the action potential conduction time taken and conduction distance increased proportionally with age; conversely the conduction velocity decreased with age. We have shown ageing induces degenerative changes in action potential conduction, contributed to by the observed loss of Cx43 protein. Our data identify Cx43 as a potential therapeutic target for quashing the age-related deterioration of the cardiac pacemaker.
