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ABE2620 Anti-CEP152 Antibody, C-Terminal

Anti-CEP152, C-Terminal, Cat. No. ABE2620, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152 kDa and has been tested in Immunofluorescence and Western Blotting.

Anti-CEP152 Antibody, C-Terminal: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CEP152, KIAA90912 UniProt Number: O94986
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ABE2620
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2620
      DescriptionAnti-CEP152 Antibody, C-Terminal
      Alternate Names
      • Centrosomal protein of 152 kDa
      Background InformationCentrosomal protein of 152 kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds CEP152 and CEP192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to centrosomes. This is a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S phase and elongate throughout S and G2 phase. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of CEP135, CEP295 (Ana1 analog) and Cep152 onto daughter centrioles.CEP152 is also an important regulator of genomic integrity and cellular response to DNA damage. Overexpression of CEP152 can drive amplification of centrioles. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical 'bird-head' facial appearance. CEP152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a result of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CEP152, C-Terminal, Cat. No. ABE2620, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152 kDa and has been tested in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected CEP152 in U2OS cells showing centrosome staining (Sonnen, K.F., et. al. (2013). J Cell Sci. 126(Pt 14):3223-33; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

      Western Blotting Analysis: A representative lot detected CEP152 in cell lysates from transfected HEK293 cells (Sonnen, K.F., et. al. (2013). J Cell Sci. 126(Pt 14):3223-33).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 277 amino acids from the C-terminal half of human CEP152.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects the long isoform of human CEP152. It targets an epitope within 277 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEP152
      • KIAA90912
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~ 230 kDa observed; 195.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected CEP152 in 10 µg of U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE2620 04054839193644