Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
MABF2034
Sigma-AldrichAnti-CD31/PECAM-1.2 Antibody, clone MBC 78.2
Anti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.
More>>Anti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting. Less<<
Anti-CD31/PECAM-1.2 Antibody, clone MBC 78.2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA', PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Anti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.
Key Applications
ELISA
Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Agonist or Inhibitor
Western Blotting
Application Notes
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD31/PECAM-1.2 in Jurkat cells.
Agonist or Inhibitor Analysis: A representative lot was used in Agonist or Inhibitor applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8).
Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.2 in THP-1 cells.
ELISA Analysis: A representative lot detected CD31/PECAM-1.2 in ELISA applications (Mei, H., et. al. (2014). J Biol Chem. 289(30):20836-44).
Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.2 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8).
Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Biological Information
Immunogen
PECAM-1 purified from human platelets.
Epitope
extracellular domain
Clone
MBC 78.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone MBC 78.2 detects CD31/PECAM-1 in human cells. It targets an epitope within the IgG homology domain 6.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.2 in THP-1 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
